Applied Entomology and Phytopathology

Evaluation of sugar beet hybrids for dual resistance to Rhizomania and Rhizoctonia root rot diseases

Mahyar Sheikholeslami Aleagha; Shirin Farzadfar

Volume 91, Issue 1 , September 2023, , Pages 33-46

https://doi.org/10.22092/jaep.2023.361223.1467

Abstract

In this research, the seeds of 12 sugar beet hybrids along with four control genotypes were planted in microplot conditions. After evaluating the roots against Rhizoctonia solani AG ... Read More

First report of Beet necrotic yellow vein virus from Matricaria chamomilla L. in Iran (Short report)

Shirin Farzadfar; Reza Pourrahim

Volume 89, Issue 1 , September 2021, , Pages 131-132

https://doi.org/10.22092/jaep.2021.354728.1402

Abstract

Several chamomile plants (Matricaria chamomilla L.) showing reduced growth were observed and collected from some fields of medicinal plants in Harsin (Kermanshah province), during summer ... Read More

First report of Grapevine virus D (GVD) in Iran

Reza Pourrahim; Shirin Farzadfar

Volume 87, Issue 2 , April 2020, , Pages 309-310

https://doi.org/10.22092/jaep.2020.341977.1328

Abstract

Most virus members of the Vitivirus genus have been isolated from grapevine (Vitis vinifera), among which Grapevine virus A (GVA), Grapevine virus B (GVB) and Grapevine virus E (GVE) ... Read More

Incidence of Beet western yellows virus (BWYV) in alfalfa fields of Lorestan and Ilam provinces (West Iran) and its taxonomical place using coat protein gene

Shirin Farzadfar; Reza Pourrahim; Mozhdeh Maleki

Volume 82, No. 1 , September 2014, , Pages 37-52

https://doi.org/10.22092/jaep.2014.100198

Abstract

During 2011 and 2012, a total of 932 alfalfa leaf samples with yellows, reddening, necrosis and stunting were collected from Lorestan and Ilam provinces. Samples were tested by tissue ... Read More

Coat protein sequence analysis of Iranian Hosta virus X (HVX) isolates

Reza Pourrahim; Shirin Farzadfar

Volume 81, No. 2 , March 2014, , Pages 123-134

https://doi.org/10.22092/jaep.2014.100370

Abstract

During the spring of 2011, several symptomatic hosta plants Hosta sieboldiana with mosaic, mottling and leaf deformation were observed in commercial hosta propagation greenhouses and ... Read More

Biological and molecular characterization of two Potato virus X- isolates from Hamedan province

N. SHOKROLLAHI; R. POURRAHIM; SH. FARZADFAR; S. NAZARI

Volume 80, No. 2 , March 2013, , Pages 119-130

https://doi.org/10.22092/jaep.2013.100565

Abstract

The main potato growing areas in Hamadan province including Bahar, Razan and Kabodar-Ahang were surveyed during 2010 and 2011 to find distribution of Potato virus X. A total of ... Read More

Distribution and some biological and molecular properties of Cauliflower mosaic virus isolates from Iranian cauliflower fields

Shirin Farzadfar; Gholamhosin MOSAHEBI; Ali Ahoonmanesh; Mina KOOHI-HABIBI; Reza Pourrahim; K. OHSHIMA; Alireza Golnaraghi

Volume 75, No. 2 , March 2008, , Pages 1-25

Abstract

A survey was conducted to determine the distribution of Cauliflower mosaic virus (CaMV) in cauliflower (Brassica oleracea var. botrytis) fields. A total of 323 symptomatic cauliflower ... Read More A survey was conducted to determine the distribution of Cauliflower mosaic virus (CaMV) in cauliflower (Brassica oleracea var. botrytis) fields. A total of 323 symptomatic cauliflower leaf samples were collected during different growing seasons from 32 fields in six provinces of Iran. These samples were tested by enzyme-linked immunosorbent assay (ELISA) using specific CaMV-polyclonal antibody. Serological diagnosis was confirmed by biological and polymerase chain reaction (PCR) tests. Based on ELISA, 292 cauliflower samples (90.4%) were infected with CaMV, ranging between 63.6-100% in the surveyed fields. Biological diversity of 21 CaMV isolates from various regions and with different symptom severity was evaluated based on their reactions on turnip (Brassica rapa), jimpson weed (Datura stramonium) and kohlrabi (B. oleracea var. gongylodes) plants. These isolates caused a variety of symptoms on turnip, including local lesions followed by vein clearing, mosaic, vein banding, rugosity and stunting. The severity of systemic symptoms observed varied depending on the isolate. In contrast, symptoms produced by the same isolates in Kohlrabi plants were restricted to older leaves and were much milder. Kohlrabi plants infected by all isolates tested eventually showed recovery and became asymptomatic. Eleven isolates induced local lesions on jimpson weed, and for two isolates, these symptoms were followed by systemic infections. All 21 isolates studied, were transmitted by green peach aphid (Myzus persicae). For molecular studies, ORF VI gene of nine selected CaMV isolates were amplified using specific primers. Comparison of sequences of the amplified fragments revealed a high identity (96.9-100%) among Iranian isolates studied. Comparison of these sequences with those available at GenBank indicated the highest identities of these isolates with D/H isolate from Hungary (96.1-96.7%). Phylogenetic studies showed clustering of Iranian isolates in a separate branch, together with the non-North American isolates. The Iranian isolates were also well differentiated from other exotic isolates using Hpy99I restriction enzyme.

Evaluation of two neo-nicotinoid root absorbing insecticides for virus disease vector controlling in seed potato fields

Reza Pourrahim; Shirin Farzadfar; Hormoz Soltani; Alireza Golnaraghi; Ali Ahoonmanesh

Volume 75, No. 2 , March 2008, , Pages 89-112

Abstract

Viral diseases and their vector insects have a great concern in seed potato production. Systemic insecticides by killing the vectors reduce the incidence of the diseases especially ... Read More Viral diseases and their vector insects have a great concern in seed potato production. Systemic insecticides by killing the vectors reduce the incidence of the diseases especially in early growing stages of potato plants. In this study, the ability of seed tuber treatment with two neo-nicotinoid root absorbing systemic insecticides, thiamethoxam and imidacloprid, were evaluated to control the insect vectors and viral diseases under field conditions with natural occurrence of vector populations. The tests were conducted using a split plot RCBD design with 3 insecticides (thiamethoxam, imidacloprid and thiodicarb) as sub-treatment and 2 potato cultivars (Agria and Marfona) as main treatment in 4 replicates. The trials were performed in two locations, Bahar (Hamadan) and Firozkoh (Tehran), in 2001 and 2002. Each insecticide treatment consisted of seed tuber treatment at planting and second treatment at hilling. In seed tuber treatment, thiamethoaxam (Cruiser 350FS), imidacloprid (Gaucho 70WS) and thiodicarb (Larvin DF80) were used at 20mL, 28.5 gr and 37.5 gr ratio for 100 Kg seed tuber, respectively. In soil application at hilling stage of potatoes, thiamethoaxam (Actara 25WG), imidacloprid (Confidor 350SC) and thiodicarb (Larvin DF80) were used at 0.14 gr, 0.14 ml and 0.05 gr ratio, respectively, for each one meter of potato rows in field. Vector populations in 3 groups including aphids, leafhoppers and thrips were evaluated. Incidence of Alfalfa mosaic virus (AlMV), Potato leaf roll virus (PLRV), Potato virus M (PVM), Potato virus S (PVS) and Potato virus Y (PVY) in the plots was detected by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA). Also, Tomatospotted wilt virus (TSWV) was detected by reverse transcription followed by polymerase chain reaction (RT-PCR) using specific primers as previously described. The data of early and late season tests were statistically analyzed. The results indicated that in early and late season, seed treatment with thiamethoxam and imidacloprid reduced significantly aphid and leafhopper numbers up to 3 fold relative to no treated controls. Thrips population was reduced up to 2 times in early season and 3 times in late season. Also, the incidence of AlMV, PVM, PVS, and PVY reduced up to half in early season and one third in late season in relative to non treated controls. Incidences of PLRV and TSWV in both early and late season were one fourth and one third of no treated controls, respectively. Thiodicarb treatments had not acceptable control on vector and disease incidence. Our results showed that seed potato tuber treatment at planting with thiamethoxam (Cruiser 350FS) or imidacloprid (Gaucho 70WS) at a ratio of 20 gr and 28.5 gr. per 100 kg of tubers, respectively, with a mid season soil application of thiamethoxam (Actara 25WG) or imidacloprid (Confidor 350SC) at a ratio of 0.03 gr and 0.14ml per one meter of plant rows, would reduce the vectors populations and viral disease incidence in potato fields. The Advantages of application of systemic root absorbing pesticids in vector and virus control in seed potato fields was discused.

Volume 91 (2022-2023)

Volume 90 (2021-2022)

Volume 89 (2020-2021)

Volume 88 (2019-2020)

Volume 87 (2019-2020)

Volume 86 (2018-2019)

Volume 85 (2017-2018)

Volume 84 (2016-2017)

Volume 83 (2015-2016)

Volume 82 (2014-2015)

Volume 81 (2013-2014)

Volume 80 (2012-2013)

Volume 79 (2011-2012)

Volume 78 (2010-2011)

Volume 77 (2009-2010)

Volume 76 (2008-2009)

Volume 75 (2007-2008)

Volume 74 (2006-2007)

Volume 73 (2005-2006)

Volume 71 (2003-2004)

Volume 70 (2002)

Volume 68 (2001)

Volume 66 (1998)

Volume 65 (1997)

Volume 64 (1997)

Author = Shirin Farzadfar

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