Applied Entomology and Phytopathology

In collaboration with Iranian Phytopathological Society

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Identification of the fungal pathogens causing leaf spot on barnyardgrass (Echinochloa crus-galli) in rice paddy fields of Mazandaran

Document Type : Plant Pathology

Authors

Abstract

Barnyardgrass is the most important weed in paddy fields of northern Iran. In order to identify the fungal pathogens causing leaf spot on this dominant weed, symptomatic plants were collected from rice fields in different areas of Mazandaran province. Leaf pieces of ca 0.5×0.5 cm from the margins of diseased spots were surface sterilized in 0.5% sodium hypochlorite and washed with sterile distilled water. The pieces were placed on water agar 2% or PDA supplemented with streptomycin sulfate. All Petri dishes were incubated at 25°C in darkness. The grown fungal colonies were isolated and purified using single spore or hyphal tip methods. To determine their pathogenicity, detached-leaf assay and spraying spore suspension on whole plants in greenhouse were used. The fungal isolates which caused symptoms, considered for further studies. Morphological identification of pathogenic isolates was performed using standard keys and monographs. In addition, genomic DNA was extracted and a nuclear rDNA region was amplified. The resulting sequences were submitted to a BLAST search to find most similar sequences in NCBI GenBank. Overall, based on morphological and molecular data, the identified fungal pathogens were Alternaria alternata, Bipolaris oryzae, Bipolaris sorokiniana, Curvularia lunata and Rhizoctonia solani.

Keywords

Main Subjects

References
AHMADPOUR, A., M. DONYADOOST-CHELAN, Z. HEIDARIAN and M. JAVAN-NIKKHAH, 2011. New species of Bipolaris and Curvularia on grass species in Iran. Rostaniha, 12: 39-49. (in Persian with English summary)
BAGHERI, F. and S. NAEIMI, 2015. Alternaria alternata, a pathogen of barnyardgrass (Echinochloa crus-galli) and nutsedges (Cyperus difformis) in rice paddy fields of Mazandaran province, Iran. 2nd Iranian Mycological Congress, University of Tehran, Karaj, Iran.
BLIGTAMI, K. S., K. K. SINHA and A. A. ANSARI, 1995. Wide spread occurrence of toxigenic Alternaria in cereals and oilseeds. Indian Phytopathology, 2: 150-153.
CHEN Y. and H. W. NI, 1999. Pathogenicity of indigenous fungi to Echinochloa crusgalli and rice. Chinese Journal of Biological Control, 15: 73-76.
FARZANEH, M., M. JAVAN-NIKKHAH, K.B. FOTOUHIFAR, and H. KARIM-MOJENI, 2009. Fungi associated with leaf spots of nut sedge plants (Cyperus spp.) in north of Iran. Rostaniha, 10: 69-79. (in Persian with English summary)
GENG, R.M., Y. FU, W.M. ZHANG, J.P. ZHANG, and L.Q.YU, 2008. Efficacy and Safety of Bipolaris sorokiniana and Bipolaris coicis for the Control of Echinochloa crus-galli in Paddy Field. Chinese Journal of Rice Science, 3: 5-15.
GENG, R. M., J. P. ZHANG and L. Q. YU, 2009. Helminthosporium gramineum Rabehn f. sp. echinochloae Conidia for Biological Control of Barnyardgrass. Weed science, 57: 554-561.
GHALANDARI, M., M. A. TAJICK, S. NAEIMI and V. KHOSRAVI, 2013. Identification of Alternaria spp. isolated from rice paddy fields of Mazandaran, Iran. 1st Iranian Mycological Congress, University of Guilan, Rasht, Iran.
ISLAM, A. K. M. M. and H. KATO-NOGUCHI, 2013. Allelopathic potential of five Labiatae plant species on barnyardgrass (Echinochloa crus-galli). Australian Journal of Crop Science, 7: 1369–1374.
JANIPOUR, S., S. A. KHODAPARAST, S. A. ELAHINIA, and F. PADASHT, 2009. A survey of sclerogenic fungal pathogens of rice plant (Oryza sativa L.) In Guilan province. Journal of Plant Protection, 23: 1-8. (in Persian with English summary)
LI, J., T. WEI, A. SUN and H. NI, 2013. Evaluation of Curvularia lunata Strain B6 as a Potential Mycoherbicide to Control Barnydrgrass (Echinochloa crus-galli). Journal of Integrative Agriculture, 12: 1201-1207.
LIU, L. M., S. W. HUANG, L. WANG, E. Q. HOU and D. F. XIAO, 2014. First Report of Leaf Blight of Rice Caused by Cochliobolus lunatus in China. Plant Disease, 98: 686-686.
MANSOORI, B. and M. A. PAZHOUMAND, 2005. Field reaction of some wheat advanced lines and cultivars to common root and crown rot pathogens in Fars province. Seed and Plant Improvement Journal, 21: 81-92. (in Persian with English summary)
MIRABOLFATHY, M. and D. ERSHAD, 2006. Bipolaris, Curvularia, Drechslera and Exserohilum diseases of turfgrass in Iran. Iranian Journal of Plant Pathology, 42: 257-274. (in Persian with English summary).
MONTAZERI, M. and M. ABBASSI, 2010. Identification of fungal pathogens of important weeds in Gorgan area fields. Proceedings of 3rd Iranian Weed Science Congress, Vol. 2, 634-636.
NAEIMI, S., V. KHOSRAVI and T. TSUKIBOSHI, 2012. Occurrence of rice infection by Bipolaris sorokiniana in Iran. Iranian Journal of Plant Pathology, 47: 355-360.
NAEIMI, S., S. M. OKHOVVAT, G. A. HEDJAROUDE and V. KHOSRAVI, 2003. Sheath rot of rice in Iran. Communication in Applied Biological Science,
68: 455-912.
PADASHT, F., G. A. HEDJARUDE and S. A. ELAHINIA, 1995. Introduction of some fungal agents of rice sheath rot disease Guilan. In Proceedings of the 12th IranianPlant Protection Congress, pp. 82.
SAFARI MOTLAGH, M. R. 2010. Isolation and characterization of some important fungi from Echinochloa spp. the potential agents to control rice weeds. Australian Journal of Crop Science, 4: 457-460.
SAFARI MOTLAGH, M. R. 2011. Reaction of major weeds and some rice cultivars to Alternaria pellucida potential biocontrol agent. Plant Pathology, 4: 163-168
SAFARI MOTLAGH, M. R. and A. JAVADZADEH, 2010. Study of Alternaria pellucida as a probable mycoherbicide for controlling arrowhead (Sagitaria trifolia) in paddy fields.
SHARIFI, M. 2001. A Practical Manual of Rice Weeds of Iran. Agricultural Research, Education and Extension Organization Publication.
TOSIAH, S., J. KADIR, M. SARIAH, A. S. JURAIMI, N. P. LO and S. SOETIKNO, 2009. Survey and evaluation of native fungal pathogens for biocontrol of barnyardgrass (Echinochloa crus-galli complex). Journal of Tropical Agriculture and Food Science, 37: 119-128.
TSUKAMOTO, H., M. GOHBARA and M. TSUDA, 1997. Evaluation of fungal pathogens as biological control agents for the paddy weed, Echinochloa species by drop inoculation. Annuals of the Phytopathological Society of Japan, 63: 366-372.
YAGHOUBI, B., M. MOHAMMADSHARIFI and M. A. BAGHESTANI, 2002. Evaluation of competitive ability of indigenous and improved rice cultivars with barnyardgrass by using reciprocal yield model. Final Report, Rice Research Institute, Rasht, Iran. (in Persian with English summary)
YANDOC, C. B., R. CHARUDATTAN and D. G. SHILLING, 2005. Evaluation of fungal pathogens as biological control agents for cogongrass (Imperata cylindrical). Weed Technology, 19:19-26.
YANG, Y. K., S. O. KIM, H. S. CHUNG and Y. H. LEE, 2000. Use of Colletotrichum graminicola KA001 to control barnyard grass. Plant disease, 84: 55-59.
ZHANG, J., G. PENG, G. DUAN, Y. ZHOU, S. YANG and L. YU, 2014. Bipolaris eleusines, a potential mycoherbicide candidate for control of barnyardgrass (Echinochloa crus-galli). Biocontrol Science and Technology, 24: 839-846.
Applied Entomology and Phytopathology
Volume 84, No. 2 - Serial Number 103
March 2017
Pages 261-268
Files
Share
How to cite
Statistics