Document Type : Pest Management
Authors
Abstract
Soil samples were collected from rhizosphere of tomato and olive plants in Golestan, Gilan, Zanjan and Qom provinces (Iran). Fluorescent pseudomonas bacterial strains were isolated on King’s B medium and their phenotypic features were characterized. For all tested strains production of fluorescent pigments on King’s B medium, arginine dihydrolase, growth at four and 41 ºC and oxidase were positive. Most of them reduced nitrate and produced gelatinase but tobacco hypersensitivity reaction (HR), levan formation and pectinolytic activity on potato slices were negative for all. Filtrate liquid was obtained from 48 h culture of the fluorescent pseudomonas on King’s B broth medium. Nematicidal activity of the bacterial filtrate against fresh hatched Meloidogyn javanica juveniles was investigated under laboratory condition. Results indicated that above 60% of the tested strains showed nematicidal activity (>50% nematode mortality) under this condition. Efficacy of the representative of the most effective strains along with the nematicide, Nemacur 10G against nematode egg production, the juveniles, mature females and gall formation, plant fresh root and aboveground weight of tomato were studied under green-house condition. Under this condition total nematode eggs, juveniles, mature females and gall formation on tomato roots were decreased significantly as compared to other treatments.
The highest number of eggs,, juveniles,, mature females and gall formation on tomato roots were observed in control treatment, and the lowest were recorded in plants treated with Pseudomonas fluoresces strain 99. There was no significant differences between the treatments as far as fresh root and aboveground weight are concerned, however, they differed significantly as compared to control check treatments in which the highest fresh root and aboveground weight.
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