Study on mating types of Mycosphaerella graminicola causative agent of septoria leaf blotch of wheat using molecular markers and its importance in Iran

KOMIJANI, S.; RAZAVI, M.; AMINIAN, H.; ETEBARIAN, H. R.

Study on mating types of Mycosphaerella graminicola causative agent of septoria leaf blotch of wheat using molecular markers and its importance in Iran

Document Type : Plant Pathology

Authors

Abstract

Abstract
In order to study presence, distribution and importance of mating types of the fungus Mycosphaerella graminicola (Fückel) J. Schröt in Cohn (anamorph Septoria tritici Roberge in Desmaze.) causative agent of septoria leaf blotch, fifty eight isolates were collected randomly from wheat fields of Golestan, Mazandaran, Ardabil, Ilam, Khozestan, Kermanshah and Fars provinces of Iran, during 2005. DNA was extracted based on standard protocols. Multiplex PCR was conducted using two mating type primers viz MAT 1-1 and MAT 1-2. Results showed that 29 isolates produced MAT 1-1 idiomorph with 340 bp molecular weight and 29 isolates produced MAT 1-2 idiomorph with 660 bp molecular weight. Both idiomorphs were detected in all locations. Chi-square analysis showed that two mating types have 1:1 ratio and strongly suggests that sexual reproduction of the fungus occurs in Iran. The pathogen with both sexual and asexual reproductions will have potential to overcome rapidly the race specific resistance sources. Therefore, it would be desirable to use cultivars with race non specific resistance genes

Keywords