Document Type : Plant Pathology
Authors
Abstract
Coat protein in plant viruses plays multiple roles in all infection cycle steps and commonly used in phylogenetic studies. To order phylogenetic analysis, genetic diversity and selection forces on partial coat protein of Zucchini yellow mosaic virus isolates, seven isolates B10, B21, B22, H7, W5, Z3 and Z14 from west of Iran were sequenced of several hosts and compared to 64 isolates retrieved from the GenBank. Phylogenetic tree were constructed based on 432 nucleotides of CP gene in MEGA6 software and estimation of genetic diversity and selection forces were performed in DnaSP version 5.10.05. In phylogenetic analysis, isolates B10, B21, B22, H7, Z3 were placed into European and Iranian group whereas, W5 and Z14 were placed in distinct group with east isolates. The largest nucleotide diversity (π=0.104) was obtained for the east isolates were in compared to Iranian, European and American isolates whereas, lowest diversity (π=0.0211) were found for the American isolates. Negative selection forces in CP gene history were deduced by ratio of non-synonymous nucleotide diversity to synonymous nucleotide diversity (dN/dS) was less than one. No recombination events were detected in this part of ZYMV-CP isolates and seems that genetic diversity in this part of genome resulted from nucleotide mutations
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