Document Type : Plant Pathology
Authors
Abstract
Tomato Bacterial canker disease, firstly reported in Iran from Urmieh in West Azarbaijan province in 1988. The disease causes lesion (canker), wilting and dryness of plants, leaf and fruit spots and whole plant decline. In this study, about 160 samples of infected plants were collected and sixty-four bacterial isolates were obtained from the infected tomato plants in Golestan and West Azarbaijan during 2004-2005. All isolates identified as Clavibacter michiganensis subsp. michiganensis based on morphological biochemical characters and pathogenecity test. Isolate Cmm (NCPPB382, Germany) was used as standard. Isolates showed considerable differences in using different sources of carbon and in their electrophoretic (SDS-PAGE) profiles of soluble cell proteins. Cluster analysis dendrograms obtained based on information derived from biochemical characters and protein electrophoresis, using software NTSYS-pc version 2/o2e, UPGMA method and Jaccard′s coefficient. Three groups (clusters) with %77 similarity based on biochemical data, 5 groups with %86 similarity based on SDS-PAGE results were realized. The results indicated high correlation and complementarirty. Resultes revealed I) heterogenesis between Iranian isolates and standard Cmm (NCPPB382), II) homogenesis between Golestan and West Azarbaijan isolates, and III) presence of different populations (strains) among the isolates selected from different areas. This is the first report of tomato bacterial disease in Golestan provice of Iran.
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