Document Type : Plant Pathology
Authors
1 Scintific memeber of IRIPP
2 Scientific member of Iranain Research Institute of Plant Protection
3 Scientific member of IRIPP
Abstract
During field surveys on May 2016, suspected leaf samples of tan spot were collected from infected wheat fields in Bileh-Savar and Germi in Ardabil province and transferred to the laboratory. Suspected wheat leaves were cut into 3-4 cm long pieces, washed under tap water and were placed on wet Whatman filter paper in Petri plates, incubated at room temperature for 24 hours under fluorescent light and then for 24 hours in the dark at 16ºC. Single conidia from the leaf surface were selected and transferred to WA and then V8 / PDA and stored in the dark for 7 days. These plates were kept under nearUV for 24 hours followed by 12 hours at 16 ºC. Dark-olive color ring of fungal conidia formed around the colony with huge amount of conidiophores and conidia. Conidia were yellow, with 5-7 pseudosepta, 14-18 × 100-200 µm and the basal cells were conical. The finding descriptions were in consistence with characteristics explained for P. tritici-repentis. For pathogenicity tests, susceptible cv. Bolani was used in greenhouse conditions. Wheat seedlings were inoculated with conidial suspension of 4×103 conidia/ml at two-leaf stage. Inoculated seedlings covered with black plastic bags for 48 hours and then transferred to the greenhouse and kept for 6 days at 22 ºC with a photoperiod of 16 hours light. P. tritici-repentis isolated again from infected wheat plants. This is the first report of the occurrence of the wheat tan spot with the causal agent of P. tritici-repentis in Moghan plain of Ardabil province
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