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Phylogenetic analysis of four Iranian Cucurbit yellow stunting disorder virus isolates based on 3' terminal region of RNA1 (Short communication in English)

Document Type : Plant Pathology

Authors

Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

To study phylogenetic analysis of some Iranian isolates of cucurbit yellow stunting disorder virus (CYSDV), 3' terminal region  of RNA1 (560 bp in length) of four CYSDV isolates, originated from four different locations of Iran including Boushehr, Kerman (Jiroft), Khuzestan (Dezfool) and Tehran provinces was amplified by RT-PCR using specific pair primers. The 3' terminal region of RNA 1 of four CYSDV was sequenced and compared with other available sequences in GenBank. Nucleotide sequences analysis of CYSDV isolates showed a high nucleotide sequence identity (98- 99%) among all isolates and nucleotide sequences identity among Iranian CYSDV isolates was 99%. Phylogenetic tree constructed by neighbor-joining method using the nucleotide sequence of amplified region indicated that all CYSDV isolates were placed in one group however three of four Iranian isolates including Boushehr, Khuzestan and Tehran were clustered into a distinct subgroup with respect to other isolates.

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References
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Applied Entomology and Phytopathology
Volume 85, No. 2 - Serial Number 105
March 2018
Pages 19-24
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