Molecular characterization of three Faba bean necrotic yellows virus (FBNYV) isolates, originated from chickpea in Iran

SOKHANSANJ, Y.; BANANEJ, K.; RAKHSHANDEHROO, F.; AHOONMANESH, A.

doi:10.22092/jaep.2018.120365.1205

Document Type : Plant Pathology

Authors

¹ PhD. Student ; Department of Plant Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Professor, Department of Plant Virus Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

³ Assistant Professor,Department of Plant Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran

⁴ Professor, Foulad Institute Technology (FIT), Foulad-shahr, Isfahan-Iran

https://doi.org/10.22092/jaep.2018.120365.1205

Abstract

Yellowing is one of the most prevalent symptoms in chickpea fields, worldwide and Iran. Faba bean necrotic yellows virus (FBNYV, genus Nanovirus, family Nanoviridae) is associated with the yellowing symptoms in the chickpea fields. During the survey of the Lorestan and Kermanshah provinces in May 2015-2016, chickpea plants showing yellowing symptoms were collected and total DNAs were extracted. The samples were checked for nanovirus infection by PCR, using nanovirus-specific degenerate primers. Total DNA preparations from the nanovirus-positive samples were used for RCA, using φ29 polymerase and restriction endonuclease Aat II digests yielding products of ~1kb corresponding to linearized nanovirus DNA components. FBNYV genomic components were amplified using RCA products and specific primers of eight different U1, M, N, C, S, R, U2, and U4 components. The amplified fragments were purified and sequenced. Nucleotide sequences of the eight different components of three Iranian isolates of FBNYV (Lor-28, Lor-1, and Ker-21) were compared with FBNYV sequences in GenBank. Sequence comparison indicated that Iranian isolates of FBNYV are most similar to Azerbaijan isolates of FBNYV: FBNYV-[AZ; 12], and FBNYV-[AZ; 13.5]. Phylogenetic analyses of nucleotide and deduced amino acid sequences of DNA-S, C, and U1 revealed that Iranian FBNYV isolates clustered with Azerbaijan isolate FBNYV-[AZ;12 ](group II), while DNA-R, DNA-M, DNA-N, DNA-U2, and DNA-U4 clustered with FBNYV-[AZ; 13.5], (group I). Considering the genomic differences between two Azerbaijan isolates, which can be expected among Iranian isolates. These results may provide evidence of the reassortment occurrence among Iranian and Azerbaijan FBNYV isolates.

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Main Subjects

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Applied Entomology and Phytopathology

Molecular characterization of three Faba bean necrotic yellows virus (FBNYV) isolates, originated from chickpea in Iran

References

References

MAKKOUK, K. and S. KUMARI, 2009. Epidemiology and integrated management of persistently transmitted aphid-borne viruses of legume and cereal crops in West Asia and North Africa Virus Research Vol.141 (2), Pages, 29-218.

Volume 86, No. 1 - Serial Number 106
September 2018
Pages 75-90

Molecular characterization of three Faba bean necrotic yellows virus (FBNYV) isolates, originated from chickpea in Iran

References

References

MAKKOUK, K. and S. KUMARI, 2009. Epidemiology and integrated management of persistently transmitted aphid-borne viruses of legume and cereal crops in West Asia and North Africa Virus Research Vol.141 (2), Pages, 29-218.

Volume 86, No. 1 - Serial Number 106September 2018Pages 75-90

Volume 86, No. 1 - Serial Number 106
September 2018
Pages 75-90