In collaboration with Iranian Phytopathological Society

Document Type : Pest Management

Authors

1 Department of Plant Protection, Faculty of Agricultural Sciences and Food Industrial, Science & Research Branch of Islamic Azad University, Tehran- Iran

2 Department of Plant Protection, Agricultural and Natural Resources Research Center of Golestan Province, Gorgan, Iran

3 Dipartimento di Scienze e Tecnologie Fitosanitarie, Sez. Patologia VegetaleUniversità degli Studi di CataniaCataniaItaly

4 Department of Mycology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

Abstract

Using natural plant products has become more important as viable alternatives to control postharvest diseases. The effect of four essential oils of medicine plants including Thymus vulgaris, Cuminum cyminum,Allium sativum and Aloe vera were tested (100, 200, 300, 400ppm) in vitro and in vivo (200,300,400 ppm) for their fungal inhibitory percentage and antagonistic properties against Penicillium digitatum. Plant essential oil extracted by hydro distillation using a Clevenger-type, and minimal inhibition concentrations (MIC) was determined using agar dilution method. In vitro inhibitory effects of the plant extracts showed maximum percent inhibition (99.95%) by thyme extract, which also had the best MIC value against P. digitatum. GC-MS analysis of the thyme oils extract led to the identification of 22 different components which carvacrol (2.88%), 60.18% thymol (60.18%), linalool (4.22%), γ-terpinene (6.39%)  were found to be the main constituents in T. vulgaris. Thyme essential oil inoculated into wounds with the pathogen stimulated the orange to increase production of phenol compound, catalase and peroxidase activity. Thus, different concentrations of the thyme essential oil are effective on controlling the producing agent of the green mold fungus significantly, and induce resistance to orange by effect on defense enzyme.
 
 

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Main Subjects

 Abd-Alla, M. S., Atalia, K. M. and El-Sawi, M. A. M. 2001. Effect of some plant waste extracts on growth and aflatoxin production by Aspergillus flavus, Annals Agrie. Sci, No. 46: 579-592.
Abdolahi, A., Hassani, A., Ghosta, Y., Javadi, T. and Meshkatalsadat, M. 2010. Essential oils as control agents of postaharvest Alternaria and Penicillium rots on tomato fruits, Journal of Food Safety, No. 30: 341–352.
Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Noorbakhsh, H., Riazi, F., Jajarmi, A. and Tabatabaei Yazdi, F. 2016. Study of the Antibacterial Activity of Methanolic and Aqueous Extracts of Myrtus communis on Pathogenic Strains Causing Infection,  Zahedan J Res Med Sci, No.18(2):e5989
Askarne, L., Talibi, I., Boubaker, H., Boudyach, E.H., Msanda, F., Saadi, B., Serghini, M.A., Ait Ben and Aoumar, A.  2012. In vitro and in vivo antifungal activity of several Moroccan plants against Penicillium italicum, the causal agent of citrus blue mold, Crop Prot,  No. 40, 53–58.
Azizi, M., Farzad, S., Jafarpour, B., Rastegar, MF. and Jahanbankhsh, V. 2008. Inhibitory effect of some medicinal plants essential oils on post harvest fungal disease of citrus fruits, International Symposium on The Role of Postharvest Technology in the Globalisation of Horticulture, No. 768:279-286.
Ballester, A. R., Lafuente, M. T., and GonzálesCandelas, L. 2006. Spatial study of antioxidant enzymes, peroxidase and phenylalanine ammonialyase in the citrus fruit-Penicillium digitatum interaction, Postharvest Biology and Technology, No.39, 115-124.
Burt, S. 2004. Essential oils: Their antibacterial properties and potential application in foods a review, International Journal of Food Microbiology, No.94, 223-253.
Deepak, S., Niranjan-Raj, S., Shailasree, S., Kini,R.K., Boland, W., Shetty, H.S. and Mithöfer, A. 2007. Induction of resistance against downy mildew pathogen in pearl millet by a synthetic jasmonate analogon, Physiological and Molecular PlantPathology, No. 71(1): 96-105.
Droby, S., Wisniewski, M., Macarisin, D. and Wilson, C. 2009. Twenty years of Post harvest biocontrol research: is it time for a new paradigm, Postharvest Biology and Technology, No. 52, 137e145
Du, Z, and Bramlage, WJ. 1995. Peroxidative activity of apple peel in relation to development of post storage disorders. HortScience, No. 30(4):205-209.
Eckert, J.W. and Brown, G.E. 1986. Postharvest citrus diseases and their control. In Wardowski, W.F., Nagy, S., Grierson, W. (Eds). Fresh Citrus Fruits, p. 315-360. New York: Van Nostrand Reinhold Company Inc.
Ghassan, J. Kanan, R. and Al-Najar, A. 2007.In vitro Antifungal Activities of Various Plant Crude Extracts and Fractions against Citrus post-harvest Disease Agent Penicillium digitatum, Jordan Journal of Biological Sciences, No.1(3): 89-99.
Gong Y, Toivonen PM, Lau O, Wiersma PA .2001. Antioxidant system level in ‘Braeburn’ apple is related to its browning disorder. Botanical Bulletin of Academia Sinica, No. 42: 259-264
Gull, I., Saeed, M., Sahukat, H., Aslam, S.M., Samra, Z.Q. and Athar, A.M. 2012. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Annals of Clinical Microbiology and Antimicrobials, No.11:1-8.
Ismail, M. A. and J. X. Zhang, 2004. Post-harvest citrus diseases and their control. Outlooks Pest Manag, No.15: 29-35.
Janisiewicz, W. J. and Korsten, L.2002.Biological control of postharvest diseases of fruits. Annual Review of Phytopathology No.40:411–41.
Perciva,l, GC. 2001. Induction of systemic acquired disease resistance in plants: potential implications for disease management in urban forestry. Journal of Arboriculture, No. 27(4):181-192
Lagrimini, L. M. and Rothstein, S .1987. Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and Tobacco Mosaic Virus infection. Plant Physiology, No. 84(2):438-442
Liochev, SI .2013. Reactive oxygen species and the free radical theory of aging, Free Radical Biology and Medicine, No. 60:1-4.
Liu, X., Wang, LP., Li, YC., Li, HY., Yu, T. and Zheng, XD.2009. Anti-fungal activity of thyme oil against Geotrichum citri-aurantii in vitro and in vivo. J Appl Microbiol, No.107:1450-1456.
Mancini, E., Senatore, F., Del Monte, D., De Martino, L., Grulova, D. and Scognamiglio, M. 2015.Studies on chemical composition, antimicrobial and antioxidant activities of five Thymus vulgaris L. essential oils. Molecules. No. 20(7): 12016-28.
Miladi, H., Slama, RB., Mili, D., Zouari, S., Bakhrouf, A. and Ammar, E. 2013. Essential oil of Thymus vulgaris L. and Rosmarinus officinalis L.:gas chromatography–mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens. Nat Sci, No. 5(6): 729-39.
Mohammadifar, M., Tavakol Norabadi, M., Hasanzadeh, M., Dashtipoor, M., Etebarian, HR. and Sahebani, N.2012. Study of antifungal activities of seven essential oils from some Iranian medicinal plants against various postharvest phytopathogenic fungi, Phytopathology and Plant protection, No.45 (17):2046-2056.
Mossini, S. A. G., Carla C. and Kemmelmeier, C. 2009. Effect of neem leaf extract and Neem oil on Penicillium growth, sporulation, morphology and ochratoxin a production. Toxins, No. 1: 3-13.
Obagwu J. and Korsten L. 2003. Control of citrus green and blue molds with garlic extracts. European Journal of Plant Pathology.No.109:221–225
Palou, L., Smilanick, J. L. and Droby, S. 2008. Alternatives to conventional fungicides for the control of citrus postharvest green and blue moulds. Stewart Posthar Rev No.4:1–16.
Palou, L., Usall, J., Smilanick, J. L., Aguilar, M. J. and Vin˜as, I. 2002. Evaluation of food additives and low toxicity compounds as alternative chemicals for the control of Penicillium digitatum and Penicillium italicum on citrus fruit. Pest Manag Sci, No.58, 459–466.
Plaza, P., Torres, P., Usall, J., Lamarca, N. and Vinas, I. 2004. Evaluation of the potential of commercial postharvest application of essential oils to control citrus decay. J Hort Sci Biotechnol,No 79, 935–940.
Porat, R., Vinokur, V., Holland, D., McCollum, G. and Droby, S. 2001. Isolation of a citrus chitinase cDNA and characterization of its expression in response to elicitation of fruit pathogen resistance. Plant Physiology, No. 158, 1585-1590
Prusky, D., Bazak M, and Ben-Arie R. 1985. .Development, persistence, survival and strategies for control of thiabendazoleresistant strains of Penicillium expansum on pome fruits. Phytopathology No.75:877–892.
Ragsdale, NN. and Sisler, HD. 1994. Social and political implications of managing plant diseases with decreased availability of fungicides in the United States. Annual Review of Phytopathology. No 32:545–57.
Šegvić Klarić, M., Kosalec, I, Mastelić, J., Piecková, E. and Pepeljnak, S .2007. Antifungal activity of thyme (Thymus vulgaris L.) essential oil and thymol against moulds from damp dwellings. Lett Applied Microbiology, No. 44: 36-42.
Sharma, R.R., Singh, D., Singh, R. 2009. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological control 50: 205-221.
Spadaro, D., & Gullino, M. L. 2004.State of art and future perspectives of biological control of postharvest fruit diseases. International Journal of Food Microbiology, 91: 185-194.
Talibi, I., Askarne, L., Boubaker, H., Boudyach, E.H., Msanda, F., Saadi, B., Ait Ben Aoumar, A. Antifungal activity of some Moroccan plants against Penicillium italicum, the causal agent of citrus blue mold. Crop Protection, NO. 35:41-46.
Tzortzaki, NG. 2009. Impact of cinnamon oil-enrichment on microbial spoilage of fresh Produce. Innovative Food Science and Emerging Technologies, No. 10: 97-102.
Wang, YS, Tian, SP, Xu, Y, Qin GZ and Yao, H.2004. Changes in the activities of pro-and anti-oxidant enzymes in peach fruit inoculated with Cryptococcus laurentii or Penicillium expansum at 0 or 20 °C. Postharvest Biology and Technology, No. 34(1): 21-28.
Yahyazadeh, M., Omidbaigi, R., Zare, R. and Taheri, H.2008. Effect of some essential oils on mycelial growth of Penicillium digitatum Sacc. World Journal of Microbiology and  Biotechnology, No.24: 1445–1450.
Yamomoto, H., Hokin, H., Tany, T. and Kadota, G.1977. Phenylalanine ammonia-lyase in relation to the corn rust resistance of oat leaves. Phytopathology, No. 90(2): 203-211.