Document Type : Plant Pathology
Authors
1 Department of Crop protection, Ferdowsi university of Mashhad
2 Department of crop protection, Ferdowsi university of Mashhad
3 Department
Abstract
Rice sheath blight disease caused by Rhizoctonia solani AG-1 IA has been recognized as a major damaging factor in many rice regions of the world. The aim of this research was investigatingon the effect of two isolates of Pseudomonas protegenes, antagonistic and inducer isolates alone and in combination with potassium silicate on expression rate and production of chitinase, β- 1, 3- gluconase and peroxidase enzymes at 0, 6, 12, 24, 48 and 72 hours after inoculation of Fajr cultivar with the causal agent of rice sheath blight disease.The amount of 1000 and 200 mg of sheath- leaf tissue was prepared as powdered in liquid nitrogen and thenin sodium acetate and phosphate buffers respectively to measure chitinase, β- 1, 3- gluconase and peroxidas eenzymes and 100 mg of the powder in RNAX-Plus solution to study the genes expression and RNA extraction. The amount of β- 1, 3- gluconas eenzyme in 72 hours and chitinase enzyme in 48 hours of infection was at maximum level. As regards, compared with other treatments, the highest level of expression of the genes for chitinase and peroxidase was observed in treatment of antagonistic bacteria in combination with potassium silicate in the early hours of the pathological process, 6 hours after inoculation with the fungal agent, and β- 1, 3- gluconase reached the maximum at 24 h, Thus this treatment recognized as the best treatment.
Keywords
ALMAGRO, L., L. V. GOMEZ ROS,S. BELCHI-NAVARRO,R. BRU, A. ROS BARCELO and M.A. PEDRENO, 2009. Class III peroxidases in plant defence reactions. Journal of Experimental Botany, 60: 377–390.
BALASUBRAMANIAN, V., D. VASHISHT, J. CLETUS, and N. SAKTHIVEL, 2012. Plant β-1,3-glucanases: Their biological functions and transgenic expression against phytopathogenic fungi. Biotechnol Lett, 34:1983–1990.
BANSODE, V. B. and S. S. BAJEKAL, 2006. Characterization of chitinases from microorganisms isolated from Lonar Lake. Indian Journal of Biotechnology, 5: 357-363.
BARNA, B., J. FODOR, B. D. HERRACH, M. POGONY and Z. Kiraly, 2012. The janus face of reactive oxygen species in resistance and susceptibility of plants to necrotrophic and biotrophic pathogens. Plant Physiology and Biochemistry, 59: 37–43.
BERGER, R. and M. REYNOLDS, 1958. The chitinase system of a strain of Streptomycesgriseus. Biochimica et Biophysica Acta, 29: 522- 534.
BHATTACHARYYA, P. N. and D. K. Jha, 2012. Plant growth-promoting rhizobacteria (PGPR): emergence
in agriculture. World Journal of Microbiology and Biotechnology, 28:1327–1350.
BOTHA, A.M., M.A.C. NAGEL, A.J. VAN DER WESTHUIZEN and F.C. BOTHA, 1998. Chitinase isoenzymes in near-isogenic wheat lines challenged with Russian wheat aphid, exogenous ethylene, and mechanical wounding. Botanical Bulletin- Academia Sinica Taipei, 39: 99-106.
BRADFORD, M.M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-54.
CAI, K., D. GAO, J. CHEN and S. LUO, 2009. Probing the mechanisms of silicon-mediated pathogen resistance. Plant Signaling and Behavior, 4: 1-3.
CHERIF, M., A. ASSELIN, and R.R. BELANGER, 1994. Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp.. Phytopathology, 84:236-242.
CHEN, C., I. LENTIK, Y. HACHAM, P. DOBREV, B. H. BEN-DANIEL, R. VANKOVA, 2014. Ascorbate peroxidase 6 protects Arabidopsis desiccating and germinating seeds from stress and mediates cross talk between reactive oxygen species, abscisic acid, and auxin. Plant Physiology, 166: 370–383.
CHITTOOR, J.M., J.E. LEACH and F.F. WHITE, 1999. In Pathogenesis-Related Proteins in Plants. Edited by DATTA, S.K. and S. MUTHUKRISHNAN, pp. 171–193. CRC Press, Boca Raton, FL.
CRUZ, M. F. A., F.A. RODRIGUES, L. R. POLANCO, C. R. S. CURVELO, K. J. T. NASCIMENTO, M. A. MOREIRA, E. G. BARROS, 2013. Inducers of resistance and silicon on the activity of defense enzymes in the soybean-Phakopsora pachyrhizi interaction. Bragantia, 72:162-172.
DATNOFF, L.E., W.H. ELMER and D.M. HUBER, 2007. Mineral Nutrition and Plant Disease, St. Paul, MN: The American Phytopathological Society.
DAWWAM, G.E., A. ELBELTAGY, H. M. EMARA, I. H. ABBAS, and M. M. HASSAN, 2013. Beneficial effect of plant growth promoting bacteria isolated from the roots of potato plant. Annals of Agricultural Science, 58: 195–201.
FAUTEUX, F., W. REMUS-BOREL, J.G. MENZIES and R.R. belanger, 2005. Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiology Letters, 249: 1–6.
FILIPPI, M.C. C., G. B. DASILVA, V. L. SILVA-LOBO, M. V. C. B. CORTES, A. J. G. MORAES and A.S. PRABHU, 2011. Leaf blast (Magnaportheoryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil. Biological control, 58: 160-166.
JAISHREE, M., J. CHITTOOR, E. LEACH, and F. FRANK, 1997. Differential induction of a peroxidase gene family during infection of rice by Xanthomonas oryzaepv. oryzae. Molcular Plant-Microbe Interaction, 10: 861–871.
KIM, S., W. KIM, E. PARK and D. CHOI, 2002. Silicon-induced cell wallsfortifcation of rice leaves: a possible cellular mechanism of enhanced host resistance to blast. Phytopathology, 92: 1095-1103.
LARSEN, J., P. CORNEJO and J. MIGUEL BAREA, 2009. Interactions between the arbuscular mycorrhizal fungus Glomus intraradicesand the plant growth promoting rhizobacteria Paenibacilluspolymyxaand P. maceransin the mycorrhizosphere of Cucumis sativus. Soil Biology and Biochemistry, 41: 286-292.
LIVAK, K.J. and T.D. SCHMITTGEN, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Δ Δ C[T]] method]. Methods, 25: 402-408.
MEW, T. W., H. LEUNG, S. SAVARY, C. M. V. CRUZ and J. E. LEACH, 2004. Looking ahead in rice disease research and management. Crop Review of Plant Science, 23: 103–127.
NISHIZAWA Y., M. SARUTA, K. NAKAZONO, Z. NISHIO, M. SOMA, T. YOSHIDA, E. NAKAJIMA, and T. HIBI, 2003. Characterization of transgenic rice plants over-expressing the stress-inducible b-glucanase gene Gns1. Plant Molecular Biology, 51:143–152.
OLIVERA, J.C., 2010. Caracterização de isolados de Xanthomonas citri subsp. malvacearum e redução da mancha-angular do algodoeiro mediada pelo silício. Master Dissertation. Universidade Federal Rural de Pernambuco. Brasil.
OU, SH, 1985. Rice Diseases, Association Applied Biology, Surrey, UK.
PASSARDI, F., D. LONGET, C. PENEL and C. DUNAND, 2004. The class III peroxidase multigenic family in rice and its evolution in land plants. Phytochemistry, 65: 1879-1893.
QIN, S., Y. J. ZHANG, B. YUAN, P. Y. XU, K. XING, and J. WANGLE, 2014. Isolation of ACC deaminase-producing habitat-adapted symbiotic bacteria associated with halophyte Limonium sinense, (Girard) Kuntze and evaluating their plant growth-promoting activity under salt stress. Plant Soil, 374: 753–766.
RESHMA, P., M.K. NAIK, M. AIYAZ, S.R. NIRANJANA, G. CHENNAPPA, S.S SHAIKH and R.Z. SAYYED, 2018. Induced systemic resistance by 2, 4- diacetylphloroglucinol positive fluorescent Pseudomonas strains against rice sheath blight. Indian Journal of Experimental Biology, 56: 207-212.
RODRIGUES, F.A., L.E. DATNOFF, 2005. Silicon and rice disease management. Fitopatologia Brasileira, 30: 457- 470.
RODRIGUES, F.A., L.E. DATNOFF, G.H. KORNDORFER, K.W SEEBOLD and M.C. RUSH, 2001. Effect of silicon and host resistance on sheath blight development in rice. Plant Disease, 85: 827–32.
RODRIGUES, F.A., D.J. MCNALLY, L.E. DATNOFF, J.B. JONES, C. LABBE, N. BENHAMOU, 2004. Silicon enhances the accumulation of diterpenoid phytoalexins in rice: a potential mechanism for blast resistance. Phytopathology, 94: 177–183.
ROZEN, S. and H. SKALETSKY, 2000. PRIMER 3 on the WWW for general users and for biologist programmers. Methods in Molecular Biology, 132: 365-86.
RYCHLIK, W, 2007. OLIGO 7 primer analysis software. Methods in Molecular Biology,402: 35-60.
SAIKIA, R., B.P. SINGH, R. KUMAR and D.K. ARORA, 2005. Detection of Pathogenesis-related Proteins– Chitinase and â-1,3-Glucanase in Induced Chickpea. Current Science, 89: 659-663.
SAKR, N. 2016. The role of silicon (Si) in increasing plant resistance against fungal diseases. Hellenic Plant Protection Journal, 9: 1-15.
SAVARY, S., L. WILLOCQUET, F.A. ELAZEGUI, N. CASTILLA and P.S. TENG, 2000b. Rice pest constraints in tropical Asia: quantification of yield losses due to rice pests in a range of production situations. Plant Disease, 84: 357–369.
SCHURT, D. A., M. F. CRUZ, K.J. NASCIMENTO, M.C. FILIPPI and F.A. RODRIGUES, 2014. Silicon potentiates the activities of defense enzymes in the leaf sheaths of rice plants infected by Rhizoctonia solani. Tropical Plant Pathology, 39: 457–463.
SEEBOLD, K.W., Jr. L. E. DATNOFF, F.J. CORREA-VICTORIA, T.A. KUCHAREK and G. H. SNYDER, 2004. Effects of silicon and fungicides on the control of leaf and neck blast in upland rice. Plant Disease, 88: 253-258.
SHARMA, N., P. TENG andF. OLIVARCE, 1990. Comparison of assessment methods for ricesheath blight disease. Philipp. Phytopathology (Philippines), 26: 20- 24.
SIMMONS, C.R. 1994. The physiology and molecular biology of plant 1, 3-â-D-glucanases and 1,3;1,4-ß-D-glucanases: Critical Review Plant Science, 13: 325- 387.
SON, J-S., M. SUMAYO, Y-J. HWANG, B-S. KIM and S-Y. GHIM, 2014. Screening of plant growth-promoting rhizobacteria as elicitor of systemic resistance against gray leaf spotdisease in pepper. Applied Soil Ecology, 73:1–8.
SPERANDIO, E. M., H. M. M do VALE, M de S. REIS, M. V. ´C. de C. B. CORTES, A. C. LANNA, and M. C. C. de FILIPPI, 2017. Evaluation of rhizobacteria in upland rice in Brazil: growth promotion and interaction of induced defense responses against leaf blast (Magnaportheoryzae), Acta Physiol Plant, 39:259-270.
TAHERI, P., and S. TARIGHI. 2010. Riboflavin induces resistance in rice against Rhizoctonia solani via jasmonate-mediated priming of phenylpropanoid pathway. Journal of Plant Physiology, 167: 201-208.
TANG, W. and R. J. NEWTON, 2005. Peroxidase and catalase activities are involved in direct adventitious shoot formation induced by thidiazuron in eastern white pine (PinusstrobusL.) zygotic embryos. Plant Physiology and Biochemistry, 43:760-769.
TORRES, M. A., D. G. J, JONATHAN, and J. L. DANGEL, 2006. Reactive Oxygen Species Signaling in Response to Pathogens. Plant Physiology, 141: 373–378.
VAN BOCKHAVEN, J., D. DE VLEESSCHAUWER and M. HOFTE, 2013. Towards establishing broad-spectrum disease resistance in plants: silicon leads the way. Journal of Experimental Botany, 64: 1281–1293.
VAN LOON, L.C., M. REP and C.M.J. PIETERSE, 2006. Significance of inducible defense related proteins ininfected plants. Annual Review of Phytopathology, 44:135–162.
VERMA, P., A. N. YADAV, S. K. KAZY, A. K. SAXENA and A. SUMAN, 2014. Evaluating the diversity and phylogeny of plant growth promoting bacteria associated with wheat (Triticum aestivum) growing in central zone of India. International Journal of Current Microbiology and Applied Science, 3: 432-447.
XAVIER- FILHA, M. S., F. A. RODRIGUES, G.P. DOMICIANO, H. V. OLIVEIRA, P. R. SILVEIRA and W.R. MOREIRA, 2011. Wheat resistance to leaf blast mediated by silicon. Australasian Plant Pathology, 40:28-38.
YEDIDIA, I., N. BENHAMOUB, Y. KAPULNIKC, and I. CHETA, 2000. Induction and accumulation of PR proteins activityduring early stages of root colonizationby the mycoparasite Trichoderma harzianumstrain T-203. Plant Physiology and Biochemistry, 38: 863−873
ZAIDI, A., E. AHMAD, M.S. KHAN, S. SAIF, and A. RIZVI, 2015. Role of plant growth promoting rhizobacteria in sustainable production of vegetables: Current perspective. Scientia Horticulturae, 193:231–239.
ZHANG, G., Y. CUI, X. DING and Q. DAI, 2013. Stimulation of phenolic metabolism by silicon contributes to rice resistance to sheath blight. Journal of Plant Nutrition and Soil Science, 176: 118–124.
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