Toxicity of Hottentotta saulcyi venom against Sesamia nonagrioides on sugarcane plants

Salabi, Fatemeh; Nazari, Mahmood; Jafari, Hadiye; Shahriari, Ali; Rezaei, Anahita

doi:10.22092/jaep.2019.122681.1240

Document Type : Agricultural Entomology

Authors

¹ Razi Vaccine and Serum Research Institute

² Department of Animal Science, Faculty of Animal science and Food Technology, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran

³ Department of Venomous Animals and Anti-venom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Ahvaz, Iran

⁴ Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

⁵ Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.22092/jaep.2019.122681.1240

Abstract

To evaluate the toxicity of Hottentotta saulcyi venom on Sesamia nonagrioides, we investigated the larval mortality, detoxification enzyme activity and histopathological alterations of the S. nonagrioides organs. Venoms were injected into the larvae at five doses (0.15µg, 0.35µg, 0.5µg, 0.75µg and 1µg; fifteen larvae per each dose) and the doses causing death were recorded during the experiment. This study demonstrated that H. saulcyi venom was able to cause external symptoms that eventually led to death of the intoxicated larvae. Moreover, this venom strongly inhibited glutathione S-transferases (GST) activity. Our data highlighted the relationship between the activity of GST enzyme and larval mortalities, so that the inactivation of this enzyme by scorpion venom may be responsible for the increasing the larval mortality. In the present study, the damage of midgut epithelium and malpighian tubules has been directly visualized and documented using histopathology methods. Clear histopathological alterations of intoxication were observed for all larvae injected with 1µg doses of venom, including a distinctive lysing and disorganizing of midgut epithelial cells that coupled with a progressive loss of the peritrophic membrane and the disappearance of microvilli.

Keywords

20.1001.1.10265007.1398.87.1.13.6

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Applied Entomology and Phytopathology

Toxicity of Hottentotta saulcyi venom against Sesamia nonagrioides on sugarcane plants

References

References

Volume 87, Issue 1 - Serial Number 108
1-12
September 2019
Pages 1-12

Toxicity of Hottentotta saulcyi venom against Sesamia nonagrioides on sugarcane plants

References

References

Volume 87, Issue 1 - Serial Number 1081-12September 2019Pages 1-12

Volume 87, Issue 1 - Serial Number 108
1-12
September 2019
Pages 1-12