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Partial Biological and Molecular Characterization of Citrus psorosis virus Infecting Satsuma mandarin (Citrus unshiu Marc) in the east of Mazandaran province

Document Type : Plant Pathology

Authors

1 Department of Plant Protection, College of Agriculture Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran

2 member of scientific board/ Mazandaran agricultural and natural resources research centre

3 Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran

Abstract

A disorder on Satsuma mandarin, Citrus unshiu (Yu.Tanaka ex Swingle) trees with bark scaling of the trunk, ring spot or mosaic pattern symptoms on mature fruits especially around the calyx and extended yellow ring spot on the old leaves observed over the recent years in Mazandaran province. The results of virus mechanical inoculation on Gomphrena globosa indicator plants and indexing on different citrus stock and biological properties of the studied isolates indicated the presence of the type B citrus psorosis virus in samples. Molecular detection of CPsV was done by using RT-PCR and specific designed primers in which a desired amplicon with a size about 600bp amplified in infected samples collected from the orchards and greenhouse. Phylogenetic analysis of the representative isolates on the basis of nucleotide sequences of the CPsV-coat protein, categorized the representative isolates into two distinct groups which the group one further divided into three subgroups. Iranian isolates were clustered in the second subgroup beside the Americans and Japanese isolates and showed the lowest identity with the isolates from Latin American and African countries

Keywords

References
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Applied Entomology and Phytopathology
Volume 87, Issue 2 - Serial Number 109
April 2020
Pages 195-207
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