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Molecular identification of the races of Fusarium oxysporum f. sp. ciceris, causal agent of chickpea wilt in western and north western provinces of Iran

Document Type : Plant Pathology

Authors

1 Department of Plant Pathology, College of Agriculture Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran

2 Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Azad University of Tehran

4 Ilam University

Abstract

Western and north western provinces of Iran have the highest potential for chickpea cultivation in Iran. Vascular wilt caused by Fusarium oxysporum f. sp. ciceris (FOC) is one of the most important diseases of this crop which distributed in chickpea cultivation areas of these provinces. The purpose of this study was to identify the pathogenic races of FOC in the western and north western provinces. Sampling was conducted on three infected locations. After isolation of the fungal isolates, identification was performed using FOC specific primers by PCR method. Out of 116 isolates identified, 94 isolates belonging to three locations were selected to recognize the fungal races. Five races including R0, R1B/C, R1A, R6 and R5 showed the highest abundance in the studied areas, respectively. Presence of five races from the eight identified races of FOC in the world in western and north western provinces of Iran indicates the high genetic diversity of this fungus in the country and distribution of the races of this fungus is independent of environmental factors.

Keywords

References
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Applied Entomology and Phytopathology
Volume 88, Issue 1 - Serial Number 110
September 2020
Pages 11-22
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