Quantification of Deoxynivalenol in wheat flour using Immunoaffinity column and High performance Liquid Chromatography

Ebadollahi, Alireza; GHAZIKHANSARI, M.

Authors

Abstract

In order to determine the amount of Deoxynivalenol (DON) in flours, five flour factories were selected among Tehran provinces' flour factories. One kilogram samples of five kinds of flour (whole, debranned, Setareh, Nool and Semolina flour) were taken from each factory randomly. The samples were transferred to laboratory and divided into 100 and subsequently into 10 gram of sub samples. Then the samples were extracted by Immunoaffinity column and analyzed with HPLC instrument which was equipped with UV detector and C₁₈ column. Mobile phase was Acetonitrile – Water in the ratio of 10-90 (V/ V).
DON was detected in all fourty flour samples. The Maximum amount of DON was in whole flour with an average level of 130.05µg/g. The minimum amount of DON was obtained in debranned flour with average level of 39.93 µg/g. Statistical analysis of data showed that there is a significant difference between whole flour and debranned and Setareh flours (P _value = 0.0171).

Keywords

References

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Applied Entomology and Phytopathology

Quantification of Deoxynivalenol in wheat flour using Immunoaffinity column and High performance Liquid Chromatography

References

References

Volume 73, No. 1 - Serial Number 79
September 2005
Pages 55-64

Quantification of Deoxynivalenol in wheat flour using Immunoaffinity column and High performance Liquid Chromatography

References

References

Volume 73, No. 1 - Serial Number 79September 2005Pages 55-64

Volume 73, No. 1 - Serial Number 79
September 2005
Pages 55-64