In collaboration with Iranian Phytopathological Society

Authors

Abstract

In order to determine the amount of Deoxynivalenol (DON) in flours, five flour factories were selected among Tehran provinces' flour factories. One kilogram samples of five kinds of flour (whole, debranned, Setareh, Nool and Semolina flour) were taken from each factory randomly. The samples were transferred to laboratory and divided into 100 and subsequently into 10 gram of sub samples. Then the samples were extracted by Immunoaffinity column and analyzed with HPLC instrument which was equipped with UV detector and C18 column. Mobile phase was Acetonitrile – Water in the ratio of 10-90 (V/ V).
DON was detected in all fourty flour samples. The Maximum amount of DON was in whole flour with an average level of 130.05µg/g. The minimum amount of DON was obtained in debranned flour with average level of 39.93 µg/g. Statistical analysis of data showed that there is a significant difference between whole flour and debranned and Setareh flours (P value = 0.0171).
 
 

Keywords

ABBAS, H. K., C. J. MIROCHA, R. J. PAWLOSKY and D. J. PUSCH, 1985. Effect of cleaning, milling and baking on deoxynivalenol in wheat. J Applied and Environmental Microbiology. Vol. 50, No. 2: 482-486.
Anonymous, 1993. Food and Drug Administration, Maximum concentration of vomitoxin in grains and grain by- products. USA.
BHAT, R. V., 1985. Thin Layer chromatography of mycotoxins. J. Chromatography. 334. 212–215.
CHAHILL, LM., SC. KRUGER, BT. McALICE, CS. RAMSEY, R. PRIOLI and
B. KOHN., 1999. Quantification of deoxynivalenol in wheat using an immunoaffinity column and liquid chromatography. J. of chromatography A. 859: 23-28.
DARAIE, B., H. YAZDANPANAH, M. SHAKER-ARDEKANI, A. SHAFAHATIE,
H. RASEKH, 1998. Occurrence of mycotoxins in different kinds of biscuite. Pajohandeh 11: 113-117 (in Persian).
JELINK, CF, AE POHLAND, GE WOOD., 1989. Worldwide occurrence of mycotroxins in foods and feeds an update. J. Assoc of anal Chem. 72: 223-230.
KOTAL, F., Z. RADOVA, 2002. A Simple method for determination of deoxynivalenol in cereal and flours. Czech J. Food Science. Vol 20, No. 2: 63-68.
PERAICA, M., B. RADIC, A. LUCIC and M. PAVLOVIC, 1999. Toxic effect of mycotoxin in humans. Bulletin of the world health organization. 77 (9): 754-66.
PESTKA, J. J., G. S. BONDY, 1990. Alteration of Immune function following dietary mycotoxin exposure. Can J. Phisiol. Pharmacol 68: 125.
RAJABZADEH, N., 2002, Technology of Cereals. (Vol. 1), Tehran university Pub. pp 22-55 , 133-252. (in Persian).
SCOT, P. M., 1990. Trichothecenes in grains. Cereal Food World. 35: 661-666.
STRATTON, O. W., A. R. ROBINSON, H. C. SMITH, L. KITTLESEN, M. BARBOUR 1993. Levels of five mycotoxins in grains harvested in Atlantic Canada as measured by performance liquid chromatography. Arch Environ. Cantam. Toxico l.24,
399-409.
ZAMANIZADEH, H., H. KHORSANDI, 1995. Occurrence of Fusarium species and other mycotoxins in wheats in Mazandaran province. J. of Plant diseases, Vol 31,
pp: 23-31(in Persian).