Production of specific monoclonal antibody to Xanthomonas translucens pathovars, the causal agents of bacterial leaf streak of cereals (Short comuincation)

Alizadeh Ali Abadi, Ali; BRAGARD, C.

doi:10.22092/jaep.2021.353583.1390

Document Type : Plant Pathology

Authors

Ali Alizadeh Ali Abadi ¹
C. BRAGARD ²

¹ Associate Professor of Plant pathology Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran

² Faculty of Bioscience Engineering, Catholic University of Louvain, Louvain-la-Neuve, Belgium

https://doi.org/10.22092/jaep.2021.353583.1390

Abstract

In order to be used in studies related to the production of a kit for detection of the causal agent of bacterial leaf streak of cereals, in contaminated wheat and barley seeds, a hybridoma cell line (named L-B7) secreting monoclonal antibody to Xanthomonas translucens pv. translucens by fusing SP2/0 myeloma cells with splenocytes from immunized BALB/c mice with one strain of this bacteria (UPB684 isolated from barley in Iran), were produced. The monoclonal antibody produced, reacted positively with X. c. pv. undulosa (15 strains) and pv. translucens (15 strains). Strains from other pathovars (X. c. pv. graminis and pv. poae) gave a negative reaction. Antibody potency was assessed by testing in ELISA. Whole cells were used both as immunogen and as antigen in ELISA tests. This antibody could be used to differentiate Xanthomonas translucens pathovars, causing bacterial leaf streak of cereals, from other pathovars, as well as in the process of producing a detection kit.

Keywords

20.1001.1.10265007.1400.89.2.11.0

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Applied Entomology and Phytopathology

Production of specific monoclonal antibody to Xanthomonas translucens pathovars, the causal agents of bacterial leaf streak of cereals (Short comuincation)

References

References

Volume 89, Issue 2 - Serial Number 113
March 2022
Pages 263-268

Production of specific monoclonal antibody to Xanthomonas translucens pathovars, the causal agents of bacterial leaf streak of cereals (Short comuincation)

References

References

Volume 89, Issue 2 - Serial Number 113March 2022Pages 263-268

Volume 89, Issue 2 - Serial Number 113
March 2022
Pages 263-268