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New species of Sphaerulina on Populus from Iran

Document Type : Plant Pathology

Authors

1 Scientific staff, Plant Disease Department, Iranian Research Institute of Plant Protection, Agricultural Research,Education and Extension Organization, Tehran, Iran.

2 Associated Prof. at Iranian Research Institute of Plant Protection, Agricultural Research Education And Extention Organization

3 Academician Prof of mycology, Botanic Department, Yerevan State University, Ervan, Armenia.

Abstract

In the framework of study on Septoria and Septoria-like species in Iran, a new species Sphaerulina osypianiae sp. nov. on Populus sp. is described using morphological and rDNA ITS sequencing data.
DNA extraction, amplification and sequencing were performed using methods and protocols mentioned by Azimi (2011). The resulting 511-bp sequence obtained from isolate 52935 was deposited in GenBank. The sequence shared 99% identity with Mycosphaerella populi (=Sphaerulina frondicola) sequences available in GenBank (see table 1 for identities). NJ tree generated using TreeView Display Option in NCBI BLAST, supported Sphaerulina species on Populus from Iran as a distinct species from all other Sphaerulina species sequences on Populus. In the framework of study on Septoria and Septoria-like species in Iran, a new species Sphaerulina osypianiae sp. nov. on Populus sp. is described using morphological and rDNA ITS sequencing data.
DNA extraction, amplification and sequencing were performed using methods and protocols mentioned by Azimi (2011). The resulting 511-bp sequence obtained from isolate 52935 was deposited in GenBank. The sequence shared 99% identity with Mycosphaerella populi (=Sphaerulina frondicola) sequences available in GenBank (see table 1 for identities). NJ tree generated using TreeView Display Option in NCBI BLAST, supported Sphaerulina species on Populus from Iran as a distinct species from all other Sphaerulina species sequences on Populus.

Keywords

References
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Applied Entomology and Phytopathology
Volume 89, Issue 2 - Serial Number 113
March 2022
Pages 257-262
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