Document Type : Plant Pathology
Authors
- Elham Mousavi Jafaripour 1
- Mozhdeh Doosti 2
- Reza Sadeghi 3
- Nargues Falahi Charkhabi 4
- Masoud Ahmadzadeh 1
1 Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran
2 Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Tehran, Tehran, Iran Research and Development Manager, Razh fadak company, Mehrshahr, Karaj,
3 , Department of Entomology and Plant Pathology, College of Agricultural Technology, Univers, Department of Entomology and Plant Pathology, College of Agricultural Technology, University College of Agriculture & Natural Resources, University of Te
4 Blvd. Imam Reza
Abstract
White button mushroom (Agaricus bisporus) has a special place in the food basket of the people worldwide because of its high nutritional value and medicinal properties. Therefore, it’s cultivation is expanding in the world. In order to identify the bacterial species associated with brown spot disease symptomatic samples were collected from edible mushrooms farms in Alborz province. including color change in the lateral margins and surface of the cap, wide spots, watery and sunken on the surface of the cap, small needle-shaped and brown spots on the cap and necrosis of the middle part and the base of edible mushroom. In addition, healthy mushrooms were sampled to isolate biocontrol bacterial agents. Sixty-one bacterial isolates were isolated among which 19 isolated were pathogenic in edible mushrooms Nine isolates were isolated from healthy mushrooms that were not pathogenic on edible mushrooms and their inhibition mechanisms were investigated. Biochemical and molecular tests were performed to identify pathogenic bacteria and endophytes. Based on the 16S rDNA sequence analysis bacterial isolates were classified into the pathogenic genera including Pseudomonas, Chryseobacterium, Ewingella, Brucella and Klebsiella and non-pathogenic genera including Bacillus velezensis, Kocuria rhizophila, Bacillus wiedmannii.
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