Document Type : Pest Management
Authors
1
1- Assistant Professor, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
2
2- Researcher, Plant Protection Research Department, Golestan Agricultural Research, Education and Extension Center, AREEO, Gorgan, Iran.
10.22092/jaep.2025.367975.1539
Abstract
Using agro-wastes is a good alternative for developing low-cost carriers for formulation of Trichoderma-based bio-products. Different carbon and nitrogen sources (rice bran, wheat bran, barley bran, Pith, Sugarcane pith, yeast extract, soybean extract, and urea) used for biomass production of T. harzianum K18 isolate. Out of 15 different substrates tested, the substrate containing wheat bran and yeast extract was the best substrate for T. harzianum k18 produced a high density of propagules (8.9 × 107 cfu/g) after 21 days. For the T. harzianum K18 formulation, some adjuants (including glycerol and carboxymethyl cellulose) added to carriers (kaolin and perlite). At the end of six months of storage period, the kaolin-based formulation with 6.51 and 6.07 log/cfu propagules per gram showed the highest viability of T. harzianum k18 in formulations at 4˚ C and 24˚ C, respectively. The best formulations based on T. harzianum K18 viability at formulations selected for greenhouse studies. 24 hours later than plants spraying with the formulations, the plants incubated with the pathogen spores and after five, 10 and 20 days of incubation, the severity of the disease evaluated. The disease severity at treatment with T. harzianum k18 formulation was 45.42 %, less than the control. Finally, it seems there is a possibility of biological control of cucumber powdery mildew using the microbial formulations of T. harzianum K18.
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