Optimization of antifungal metabolite surfactin production by Bacillus velezensis UTB96

Document Type : Pest Management

Authors

1 Dept. of Plant Protection, College University of Agriculture and Natural Resources, University of Tehran

2 Dept. of Plant Protection, College University of Agriculture and Natural resources, University of Tehran

3 Institute of Food Science and Biotechnology. University of Hohenheim

10.22092/jaep.2025.366438.1522

Abstract

Surfactin is one of the most effective bacterial cyclic lipopetides mainly produced by different species of Bacillus that has antifungal activity.  This compound are considered for using in various industries, medical and pharmaceutical and agricultural as an effective antibiotic for pest and disease management. Mostly the production of secondary metabolites in microorganisms is strongly influenced by physical and chemical conditions. In the current study, effect of some factors on surfatin production of B. velezensis UTB96 were evaluated. This strain is able to reduce the surface tension of the water from 72 mN/m to 30 mN/m. In this research we evaluated the production of surfactin by B. velezensis UTB96 as a potent producer. The strain was cultured in two different media. The effect of different incubation time including 24, 48 and 72 hours, different temperature including 25 and 37 ˚C, and also, different sources of nitrogen and finally different concentration of microelements surfactin production has been evaluated using HPLC analysis. HPLC analysis indicated that surfactin considerably produced at the optimum temperature of 37˚C for a 48h-incubation period. Regarding media components, it was found that the optimum nitrogen source was NH4NO3 (50 mM) and also, the optimum mineral slats were MnSO4 (0.01 mM), FeSO4 (4 uM). These results tend to suggest that B. velezensis UTB96 might be an appropriate strain to produce surfactin in bioreactor fermentation for large-scale production in order to apply for controlling A. flavus and to reduce its dangerous mycotoxin, aflatoxin as well. It was also revealed that the extracted cyclic lipopeptides could inhibit the growth of the fungus Aspergillus flavus at the concentration of 1mg/ml.

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Main Subjects

References

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Applied Entomology and Phytopathology
Volume 93, Issue 1 - Serial Number 120
September 2025
Pages 13-25

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