Document Type : Pest Management
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Abstract
The present study was conducted to determine the efficacy of Trichoderma asperellum Volatile Organic Compounds (VOCs) in suppression of mycelial growth and conidial germination of Botrytis cinerea and also in control of Botrytis fruit rot of strawberry. Significant reduction (P< 0.01) in colony diameter, percent of conidial germination and length of germ tubes of B. cinerea was observed in treatments exposed to VOCs of 5 days- old T. asperellum compared control. The incidence and severity of Botrytis fruit rot of strawberry was also significantly (P< 0.01) reduced by exposure to the VOCs of 5 day old T. asperellum cultures. Solid phase microextraction coupled to gas chromatography–mass spectrometry (SPME-GC-MS) technique was used to study the VOCs profile of T. asperellum. Thirty four single VOCs were identified by comparing the mass spectra of the individual VOC with those for the standard compounds deposited in the database of the NIST/EPA/NIH library (NIST05) and interpretation of their fragmentation pattern and their respective molecular ions. Among them, six compounds including isobutyric acid, 1,3,5,7-cyclooctatetraene, dimethyl sulfide, para-menta-6,8,dien-2-ol-acetate, phenyl ethyl alcohol and 1-butanol, 3-methyl-, acetate were the most abundant. Synthetic compounds of isobutyric acid and dimethyl sulfide were the most effective compounds among these compounds in suppression of mycelial growth and conidial germination of B. cinerea with IC50 of 26.5 and 10 μl/ liter, respectively.
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