Some cryoprotectants of overwintering larvae of rice stem borer Chilo suppressalis Walker (Lep.: Pyralidae) collected from northern parts of Iran

Atapour, Maryam; Moharramipour, Saeid; Barzegar, Mohsen; Khani, Abbas

Authors

Department of Entomology, Faculty of Agriculture, Tarbiat Modares University

Abstract

The rice stem borer, Chilo suppressalisWalker overwinters as diapausing last instar larvae in which some carbohydrates were shown to be changed. To determine the presence of these compounds and their changes in diapause stage, mature overwintering larvae were collected from overwintering sites in rice fields during October 2004 to March 2005. Cryoprotectants and glycogen were measured by high-performance liquid chromatography (HPLC) and UV-Vis spectrophotometer respectively.
Glycerol was identified as the major antifreeze compound and trehalose as the main sugar that both changed significantly during the different months. The lowest and highest content of glycerol was reported on October and January (1.21±0.18 and 15.53±3.7 mg/g of larval fresh weight respectively). In contrast of glycerol, trehalose showed the highest concentration on November (7.00±0.61 mg/g) and the lowest on January (2.79±0.57 mg/g); however, its content was nearly constant from November to late winter. Despite of negative correlation between glycerol and trehalose, this was not significant. Glycogen content was decreased as glycerol content was increased and there was negative and significant correlation between them (r =0.964; P<0.01)showing the interconversion of glycogen to glycerol during the overwintering period. It is concluded that glycerol may be more influential in cold hardiness of C. suppressalis in north parts of Iran and the insect’s diapause could be initiated on late November and terminated on February-March.

Keywords

References

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