Preparation of nematicides formulation from Achillea wilhelmsii for controlling root knot nematodes (Meloidogyne incognita)

Document Type : Pest Management

Authors

Iranian Research Institute of Plant Protection

Abstract

Foliage of Achillea wilhelmsii C. Koch. were dried in the shade and room temperature (about 25°C), powdered and extraction was done in the Soxhlet extractor. Water soluble and non-water soluble components of the extract were separated by Dichloromethane/water. In addition, Tween 85 was added to the extract and EC formulation of 5% was made. Results of the laboratory experiments showed good stability of the formulation. The toxicity of formulation to the root-knot nematode, Meloidogyne incognita, was studied in laboratory and pot experiments. Application rates of formulation were 0.125, 0.25, 0.5, 1, 2, 4, 8 and 16 percent for the laboratory experiment in order to determine their effects on motility and mortality of second-stage juveniles (J2) after 24 and 48 h. In pot experiment, 0.125, 0.25, 0.5, 1, 2 and 4 percent of the formulation were applied on nematode activity and plant growth components of tomato (Solanum lycopersicum). Exposure time was significantly (p<5%) affected J2 mortality. J2 mortality was 49.9% for concentration of 4%. It was 100% for the concentrations of 8% and 16%, after 48 h. In pot experiment, all of the concentrations significantly reduced the nematode parameters and overall population. Treatment of 4% of formulation was toxic to tomato plants in comparison to control.  In general, concentrations of the 0.50%, 1% and 2% of the formulation were at par and the bests in controlling root knot nematode and improved tomato growth parameters in the pot condition compare with the control.

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References

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