بررسی همراهی پروکاریوت‌های بیماری‌زای گیاهی محدود به آوند آبکشی با علایم بدشکلی و رنگ‌گیری غیرطبیعی میوه در درختان نارنگی سیاهو (cv. Siyahoo Citrus reticulata)

نوع مقاله : بیماری‌شناسی گیاهی

نویسندگان

1 عضو هیئت علمی بخش تحقیقات گیاهپزشکی مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی فارس

2 عضو هیئت علمی بخش تحقیقات گیاهپزشکی مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی هرمزگان

3 کارشناس حفظ نباتات سازمان جهاد کشاورزی هرمزگان- فارغ التحصیل بخش گیاه‌پزشکی، دانشکده علوم زراعی، دانشگاه کشاورزی و منابع طبیعی

4 بخش ویروس شناسی گیاهی دانشکده کشاورزی دانشگاه شیراز

5 بخش تحقیقات گیاهپزشکی،مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی فارس

چکیده

در دهه اخیر در درختان نارنگی منطقه­ سیاهو در استان هرمزگان علایم بدشکلی و رنگ­گیری غیرطبیعی در میوه­ها مشاهده شده که خسارت اقتصادی زیادی را به باغداران وارد کرده است. به­منظور بررسی­ نقش پروکاریوت­های محدود به آوند آبکشی شامل Candidatus Liberibacter asiaticus، Candidatus Phytoplasma spp. و Spiroplasma citri با این علایم، از 40 درخت دارای علایم و پنج درخت بدون علایم، نمونه­برداری از میوه­، دم­میوه و برگ­های اطراف میوه انجام شد. استخراج DNA کل از دم­میوه و رگبرگ میانی انجام و ردیابی مولکولی این بیمارگرها با آزمون PCR با استفاده از آغازگرهای اختصاصی انجام شد. در همه­ درختانی که در میوه آن‌ها علایم رنگ­گیری غیرطبیعی و بدشکلی وجود داشت، باکتری Ca. L. asiaticus، عامل فرم آسیایی بیماری هوانگ­لونگ­بینگ مرکبات (میوه‌سبز مرکبات)، ردیابی شد. در نمونه­های دارای علایم، نتیجه ردیابی فیتوپلاسما  منفی بود و تنها در چهار درخت، Spiroplasma citri، عامل استابورن مرکبات، به همراه Ca. L. asiaticus ردیابی شد. در هیچ یک از پنج درخت بدون علایم، بیمارگرهای نامبرده ردیابی نشدند. با تعیین توالی برخی از قطعات تکثیر شده در PCR، جستجو با برنامه­ بلاست و آنالیز تبارزایی، وجود گونه­ Ca. L. asiaticus در درختان دارای علایم تایید شد. نتایج به­دست آمده نشان داد که باکتری Ca. L. asiaticus با علایم رنگ­گیری غیرطبیعی و بدشکلی میوه در درختان نارنگی سیاهو همراه می­باشد و این نشان­دهنده­ گسترش بیماری میوه‌سبز مرکبات در این منطقه است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of the association between phloem-limited plant pathogenic prokaryotes and fruit malformation and abnormal coloration symptoms in Siyahoo mandarin (Citrus reticulata cv. Siyahoo) trees

نویسندگان [English]

  • Mohammad Mehdi Faghihi 1
  • Abolnabi Bagheri 2
  • Mjeed Askari seyahooei 2
  • Siavash Samavi 3
  • Mohsen Amiri Mazraie 4
  • NazaninSadat Ebadi 5
1 Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center, Zarghan, Iran.
2 Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Bandar Abbas, Iran
3 Department of Plant Pathology, College of Agronomy Sciences, Sari Agricultural and Natural Resources University, Sari, Iran
4 : Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran
5 Plant Protection Research Department, Fars Agricultural and Natural Resources Research and Education Center
چکیده [English]

 Over the past decade, symptoms of abnormal fruit coloration and deformation have been observed in mandarin (Citrus reticulata) trees in the Siyahoo region of Hormozgan Province, resulting in significant economic losses due to reduced marketability. To investigate the potential involvement of phloem-limited prokaryotes (Candidatus Liberibacter asiaticus, Candidatus Phytoplasma spp., and Spiroplasma citri), forty symptomatic and five asymptomatic trees were examined. Sampling was primarily conducted on the peduncles of symptomatic fruits, and DNA was extracted from both peduncles and midribs. Molecular detection of the target pathogens was performed using PCR assays with specific primer sets. Results revealed that Ca. Liberibacter asiaticus, the causal agent of the Asian form of citrus huanglongbing (HLB), was consistently identified in all trees exhibiting fruit discoloration and deformation. In contrast, phytoplasmas were not detected in any symptomatic trees, while Spiroplasma citri, the causal agent of citrus stubborn disease, was identified in only four trees with symptomatic fruits and leaves. None of these pathogens were detected in the five asymptomatic trees. Sequencing of selected PCR products, BLAST search and phylogenetic analyses confirmed the presence of Ca. L. asiaticus in symptomatic trees. Overall, PCR detection demonstrated a strong association between Ca. L. asiaticus and fruit malformation and uneven coloration, confirming the presence and spread of HLB in mandarin orchards of the Siyahoo region.
 

کلیدواژه‌ها [English]

  • Citrus
  • Hormozgan
  • Huanglongbing
  • PCR

مراجع

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آفات و بیماری‌ های گیاهی
دوره 93، شماره 2
اسفند 1404
صفحه 180-195

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