نوع مقاله : بیماریشناسی گیاهی
نویسندگان
1 گروه فناوری و مدیریت تولید، پژوهشکده گل و گیاهان زینتی، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی- محلات، ایران
2 پژوهشکده گل و گیاهان زینتی ، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی.
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Corm rot and Fusarium yellowing are the major disease of gladiolus with widespread prevalence. The causal agent is the fungus Fusarium oxysporum f. sp. gladioli, which causes corm rot, leaf yellowing, and eventual plant death of gladiolus. Fusarium yellowing and wilt is a severe and highly destructive disease, causing 30–70% yield losses in gladiolus. Disease management relies on integrated strategies, including cultural, chemical, and biological methods, as well as using resistant cultivars. In this study, morphological and biological characteristics of various fungal isolates were investigated. Additionally, four pairs of universal and specific primers were employed to determine species identification, forma specialis, and the race of selected isolates. Results demonstrated that all isolates were pathogen on gladiolus cv. Oscar. Universal primers ITS and Tef enabled fungal identification at the subspecies level. Application of primer pairs FOGA/B and FOGE/F revealed that all examined isolates belonged to race 2 of the fungus. This study represents the first molecular characterization and identification of Fusarium oxysporum f. sp. gladioli (FOG) isolates from Iran.
کلیدواژهها [English]