نوع مقاله : بیماری‌شناسی گیاهی
نویسندگان
1 آزمایشگاه پروکاریوت شناسی، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
2 استادیار و محقق بخش تحقیقات گیاهپزشکی، مرکز تحقیقات کشاورزی و متابع طبیعی استان لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی،
3 محقق بخش تحقیقات گیاهپزشکی، مرکز تحقیقات کشاورزی و متابع طبیعی استان لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، خرم آباد،
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Chickpea witches’ broom disease, characterized by symptoms including yellowing, excessive branching, leaf narrowing, and shortened internodes, was first observed in 2020 in chickpea fields of Lorestan Province, Iran. Samples from symptomatic and asymptomatic chickpea plants were collected and examined for the presence of phytoplasma using biological and molecular approaches. Phylogenetic analysis and virtual RFLP patterns revealed that the phytoplasma associated with chickpea witches’ broom disease belongs to the 16SrIX group. Inoculation of healthy chickpea plants with infected scions and dodder (Cuscuta spp.) under insect-free greenhouse conditions resulted in the development of disease symptoms. Among the weed species collected from around infected fields, Galium aparine was identified as a host of phytoplasma groups 16SrIX-B and 16SrIX-C. Molecular analysis of sap-sucking insects (psyllids, leafhoppers, and aphids) collected from infected fields did not identify any candidate insect vector. Nested PCR assays showed that 38% of seedlings grown from seeds collected from infected plants were phytoplasma-positive 20 days after germination, whereas seedlings derived from asymptomatic plants were free of infection. Infected seeds produced two distinct symptom types, and biological and molecular analyses indicated the presence of different phytoplasma groups, namely 16SrIX-B and 16SrIX-C. Simultaneous infection of chickpea plants with both strains intensified symptom severity and induced necrotic reactions. These findings suggest that interactions between phytoplasma strains play an important role in determining the severity of chickpea witches’ broom disease.
کلیدواژه‌ها [English]
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