با همکاری انجمن‏‌ بیماری شناسی گیاهی ایران

نوع مقاله : بیماری‌شناسی گیاهی

نویسندگان

1 دانشجوی دکتری بیماری شناسی، گروه بیماری شناسی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانشیار گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

3 فارغ التحصیل کارشناسی ارشد بیماری شناسی گیاهی دانشگاه علوم کشاورزی و منابع طبیعی ساری

4 دانشجوی کارشناسی ارشد بیماری شناسی گیاهی دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

به منظور مطالعه‌ی گونه‌های بیماری‌زای جنس Fusarium روی میزبان‌های مختلف نمونه‌های گیاهی آلوده به بیماری‌های قارچی متعددی از استان‌های یزد، کرمان و مازندران طی سال‌های 93-1391 جمع‌آوری شد. هدف از این مطالعه، امکان سنجی گونه‌های بیماری‌زا بر اساس خصوصیات ریخت‌شناسی و مولکولی و انگشت‌نگاری ژنتیکی و فهم ارتباط بین این گونه‌ها بود. نتایج حاصل نشان داد که استفاده از تکنیک rep-PCR و ترادف حاصل از ناحیه‌ی ITS-rDNA روشی مناسب برای شناسایی گونه‌های مختلف فوزاریوم است. همچنین براساس نتایج به‌دست آمده، نمونه‌های مطالعه شده به عنوان هشت گونه‌ تشخیص داده شدند: F. brachygibbosum، F. delphinoides، F. equiseti، F. globosum، F. graminearum، F. incarnatum، F. oxysporum و F. proliferatum. گونه‌های F. brachygibbosum عامل لکه برگی روی خرزهره، F. delphinoides عامل پوسیدگی ریشه روی شبدر زینتی، F. equiseti عامل شانکر روی کرچک ، F. globosum عامل سوختگی غلاف نی، F. incarnatum عامل شانکر تاج خروس، F. oxysporum عامل پوسیدگی برگ روی آلوئه ورا و F. proliferatum به عنوان عامل شانکر روی کنجد، سوختگی برگ روی سیکاس و عامل همراه با پژمردگی آوندی روی نخل برای اولین بار از روی این میزبان‌ها از ایران گزارش می‌شوند. همچنین گونه‌ی F. delphinoides یک گونه‌ی جدید برای فلور قارچی ایران می‌باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of Genotypic and Phenotypic Characteristics of several Plant Pathogenic Fusarium Isolated on Different Hosts

نویسندگان [English]

  • H. A. MIRHOSSEINI 1
  • V. BABAEIZAD 2
  • L. HASHEMI 3
  • E. BASAVAND 4

1 Sari Agricultural Sciences and Natural Resources University

چکیده [English]

In 2011-2013, to study of plant pathogen Fusarium species on different hosts, the samples from Yazd, Kerman and Mazandaran provinces were collected. This study was purposed for identification of plant pathogen Fusarium species on the basis of morphological and molecular characteristics and relationship between of species. The samples were detected to eight different species including: F. brachygibbosum, F. delphinoides, F. equiseti, F. globosum, F. graminearum, F. incarnatum, F. oxysporum and F. proliferatum. F. brachygibbosum as causal agent of oleander leaf spot, F. delphinoides as kidney weed root rot, F. equiseti as stem canker in castor bean, F. globosum as giant cane sheet blight, F. incarnatum as stem canker on redroot pigweed, F. oxysporum as aloe leaf rot and F. proliferatum as causal agents of sesame canker, cycas leaf blight and date palm vascular wilt, for first time have reported in Iran. F. delphinoides is a new species for the mycoflora of Iran.

کلیدواژه‌ها [English]

  • ITS-rDNA
  • Morphological and molecular studies
  • rep-PCR
ALVES-SANTOS, F. M., E. P. BENITO, A. P. ESLAVA and J. M. DIAZ-MINGUEZ, 1999. Genetic diversity of Fusarium oxysporum strains from common bean fields in Spain. Applied and Environmental Microbiology No. 65: 3335-3340.
AMICUCCI, A., I. ROSSI, L. POTENZA, A. ZAMBONELLI, D. AGOSTINI, F. PALMA and V. STOCCHI, 1996. Identification of ectomycorrhizae from tuber species by RFLP analysis of the ITS region. Biotechnology Letters No. 18: 821-826.
APPEL, D. J. and T. R. GORDON, 1995. Intraspecific variation within populations of Fusarium oxysporum based on RFLP analysis of the intergenic spacer region of the rDNA. Experimental Mycology No. 19: 120-128.
BOUTATI, E. I. and E. J. ANAISSIE, 1997. Fusarium, a significant emerging pathogen in patients with hematologic malignancy: ten years’ experience at a cancer center and implications for management. Blood No. 90: 999-1008.
BURGESS, L. W., B. A. SUMMERELL, S. BULLOCK, K. P. GOTT and D. BACKHOUSE, 1994. Laboratory manual for Fusarium research. 3th ed., University of Sydney, Australia. 133 p.
DATTA, S., R. G. CHOUDHARY, M. D. SHAMIM and V. DHAR, 2011.Polymorphism in the internal transcribed spacer (ITS) region of the ribosomal DNA among different Fusarium species. Archives of Phytopathology and Plant Protection No. 44: 558-566.
DE-BRUIJN, F. J., J. W. RADEMAKER, M. SCHNEIDER, U. ROSSBACH and F. J. LOUWS, 1996. Rep-PCR genomic fingerprinting of plant- associated bacteria and computer-assisted phylogenetic analysis, pp. 497-502, In: S. Grey, M. Beth and M. G. Peter (Eds). Biology of Plant-Microbe Interaction. APS Press, USA.
EBADI, M., H. RIAHI and R. ZARE, 2013. Genetic diversity of Fusarium semitectum isolates from rice, using RAPD and REP-PCR markers. Mycologia Iranica No. 1: 14-20.
EDEL, V., C. STEINBERG, I. AVELANGE, G. LAGUERRE and C. ALABOUVETTE, 1995. Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology No. 85: 579-585.
GROPPE, K. and T. BOLLER, 1997. PCR assay based on a microsatellite-containing locus for detection and quantification of Epichloe endophytes in grass tissue. Applied and Environmental Microbiology No. 63: 1543-1550.
GUREL, F., G. ALBAYRAK, O. DIKEN, E. CEPNI and B. TUNALI, 2010. Use of rep-PCR for genetic diversity analyses in Fusarium culmorum. Journal of Phytopathology No. 158: 387-389.
HEALY, M., K. REECE, D. WALTON, J. HUONG, S. FRYE, I. I. RAAD and D. P. KONTOYIANNIS, 2005. Use of the Diversilab System for Species and Strain Differentiation of Fusarium Species Isolates. Journal of Clinical Microbiology No. 43: 5278–5280.
INGLE, A., A. KARWA, M. K. RAI and Y. GHERBAWY, 2009. Fusarium: molecular detection mycotoxins and biocontrol, pp. 85-106. In: Y. Gherbawy, R. Mach and M. Rai (Eds). Current Advances in Molecular Mycology. Science Publishers Inc., Enfield.
KAWURI, R., DN. SUPRAPTA, Y. NITTA and T. HOMMA, 2012. Destructive leaf  rot disease caused by Fusarium oxysporum on Aloe barbadensis Miller in Bali. Agricultural Science Research Journal No. 2: 295 – 301.
LESLIE, J. F. and B. A. SUMMERELL, 2006. The Fusarium laboratory manual. 3th ed., Blackwell Publishing Professional, Ames, 388 p.
LOMBARD, V. C. P. BARIL, P. DUBREUIL, F. BLOUET and D. ZHANG, 2000. Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: Consequences for varietal registration. Crop Science No. 40: 1417-1425.
MAYEK-PEREZ, N., Z. LOPEZ-CASTANEDA, M. GONZALEZ-CHAVIRA, R. GARCIA-ESPINOSA, J. ACOSTA-GALLEGOS, O. MARTINEA-DELAVEGA and J. SIMPSON, 2001. Variability of Mexican isolates of Macrophomina phaseolina based on pathogenesis and AFLP genotype. Physiological and Molecular Plant Pathology No. 59: 257-264.
MIRHOSSEINI, H. A., V. BABAEIZAD and L. HASHEMI, 2014. First report of Fusarium brachygibbosum causing leaf spot on oleander in Iran. Journal of Plant Pathology No. 96: 431-439.
NAKAMURA, H., A. KANEKO, S. YAMAOKA and M. KAKISHIMA, 1998. Differentiation of Melampsora rust species on willows in Japan using PCR-RFLP analysis of ITS regions of ribosomal DNA. Mycoscience No. 39: 105-113.
NELSON, P. E., T. A. TOUSSON and W. F. O. MARASAS, 1983. Fusarium species, an illustrated manual for identification. University Park: Pennsylvania State University Press. 193 p.
O’DONNELL, K. 1992. Ribosomal DNA internal transcribed spacers are highly divergent in the phytopathogenic ascomycete Fusarium sambucinum (Gibberella pulicaris). Current Genetics 22: 213-220.
O’DONNELL, K., D. A. SUTTON, M. G. RINALDI, K. C. MAGNON, P. A. COX, S. G. REVANKAR, S. SANCHE, D. M. GEISER, J. H. JUBA, J. A. VAN-BURIK, A. PADHYE, E. J. ANAISSIE, A. FRANCESCONI, T. J. WALSH and J. S. ROBINSON, 2004. Genetic diversity of human pathogenic members of the Fusarium oxysporum complex inferred from multilocus DNA sequence data and amplified fragment length polymorphism analyses: evidence for the recent dispersion of a geographically widespread clonal lineage and nosocomial origin. Journal of Clinical Microbiology No. 42:5109-5120.
PETERSON, S. W. and A. LAGRIECO, 1991. Ribosomal RNA sequence variation among inter fertile strain of some Gibberella species. Mycology No. 83: 397-402.
RAAD, I., J. TARRAND, H. HANNA, M. ALBITAR, E. JANSSEN, M. BOKTOUR, G. BODEY, M. MARDANI, R. HACHEM, D. KONTOYIANNIS, E. WHIMBEY and K. RALSTON, 2002. Epidemiology, molecular mycology, and environmental sources of Fusarium infection in patients with cancer. Infection Control and Hospital Epidemiology No. 23: 532-537.
RADEMAKER, J. W., B. HOSTE, F. J. LOUWS, K. KERSTERS, J. SWINGS, L. VAUTERINE, P. VAUTERINE and F. J. DE-BRUIJN, 2000. Comparison of AFLP and rep-PCR genomic fingerprinting with DNA-DNA homology studies: Xanthomonas as a model system. International Journal of Systematic and Evolutionary Microbiology No. 50: 665-677.
REDONDO, C., J. CUBERO and P. MELGAREJO, 2009. Characterization of Penicillium species by ribosomal DNA sequencing and BOX, ERIC and REP-PCR analysis. Mycopathologia No. 168: 11-22.
ROHLF, F. J. 2000. NTSYSpc, Numerical taxonomy and multivariate analysis system. Version 2.1 Exete software. Applied Biostatistics INC., NY, USA. 493-505.
SALLEH, B. 1998. Mycotoxins: their implication in animal and human health. USM Academic Imprint, 155 p.
SCHROERS, H., K. O’DONNELL, S. C. LAMPRECHT, P. L. KAMMEYER, D. A. SUTTON, M. G. RINALDI, D. M. GEISER and R. C. SUMMERBELL, 2009. Taxonomy and phylogeny of the Fusarium dimerum species group. Mycologia No. 101: 44-70.
STEINKELLNER, S., R. MAMMERLER, and H. VIERHEILIG, 2008. Germination of Fusarium oxysporum in root exudates from tomato plants challenged with different Fusarium oxysporum strains. European Journal Plant Pathology No. 122: 395-401.
TAMURA, K., G. STECHER, D. PETERSON, A. FILIPSKI and S. KUMAR, 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution No. 24: 1596-1599.
TIVANG, G., J. NIENHUIS, and O. S. SMITH, 1994. Estimation of sampling variance of molecular marker data using the bootstrap procedure. Theoretical Applied Genetics No. 89: 259-264.
TORRES, H. and D. P. KONTOYIANNIS, 2003. Hyalohyphomycoses. Pp. 252–270. In: W. E. Dismukes, P. G. Pappas and J. D. Sobel (Eds). Oxford Textbook of Clinical Mycology. Oxford University Press, New York.
TRANE, U. 1990. Grouping Fusarium section Discolor isolates by statistical analysis of quantitative high performance liquid chromatographic data on secondary metabolite production. Journal of Microbiological Methods No. 12: 23-39.
VAKALOUNAKIS, D. J. 1996. Root and stem rot of cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum. Journal of Plant Disease No. 81: 313-316.
VERSALOVIC, J., M. SCHNEIDER, F. J. DE-BRUIJN and J. R. LUPSKI, 1994. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods of Molecular and Cellular Biology No. 5: 25-40.
VERSALOVIC, J., T. KOEUTH and J. R. LUPSKI, 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genome. Nucleic Acid Research No. 19: 6823-6831.
WAALWIJK, C., R. P. BAAYENA and W. GAMS, 1996. Discordant groupings of Fusarium spp. from sections Elegans, Liseola and Dlaminia based on ribosomal ITS1 and ITS2 sequences. Mycologia 88: 361-368.
WHITE, T. J., T. BRUNS, S. LEE and J. W. TAYLOR, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. pp. 315-322. In: M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White (Eds). PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, USA.
WITHANAGE, G. S., H. MURATA, T. KOYAMA and I. ISHIWATA, 2001. Agonistic and antagonistic effects of zearalenone, an estrogenic mycotoxin, on SKN, HHUA, and HepG2 human cancer cell lines. Veterinary and Human Toxicology 43: 6-10.