با همکاری انجمن‏‌ بیماری شناسی گیاهی ایران

نوع مقاله : مدیریت آفات و بیماری‌های گیاهی

نویسندگان

1 دانشجوی دکتری گروه گیاه‌پزشکی، دانشکده علوم کشاورزی و صنایع ‌غذایی، واحد علوم‌ و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار پژوهشی بخش تحقیقات کنترل بیولوژیک، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

3 استادیار گروه گیاه پزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 دانشیار گروه گیاه‌پزشکی، دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع‌طبیعی، دانشگاه تهران، کرج، ایران

چکیده

این پژوهش با هدف، جداسازی و شناسایی قارچ‌های اندوفیت برنج به‌منظور کاربرد آن‌ها به‌عنوان جایگزین مؤثر و بی‌خطر به جای قارچکش‌های شیمیایی برای کنترل بیماری پوسیدگی طوقه با عامل Fusarium fujikuroi  انجام شد. قارچ‌های اندوفیت، از برگ، ساقه، غلاف و ریشه بوته‌های برنج شالیزارهای استان مازندران و گیلان جداسازی شد. جدایه‌های قارچی به دست آمده، ابتدا در آزمون کشت متقابل علیه سویه پُرآزار F. fujikuroi F257غربال شده و سپس سویه‌های مؤثر برای آزمون گلخانه انتخاب شدند. بذور برنج با جدایه‌های منتخب اندوفیت، تیمار و درصد وقوع بیماری در نشاها و نیز شاخص‌های رشد برنج تعیین شدند. کمترین میزان وقوع مرگ گیاهچه در نشاهای برنج تحت تیمار قارچ‌های اندوفیت
NR-R688 Chaetomium globosumو C. globosum NR-SH321  با دو درصد (4/97 درصد کنترل وقوع بیماری)،  NR-L243 Penicillium sp. و Fusarium sp. NR-L645 با شش درصد (3/92 درصد کنترل وقوع بیماری) مشاهده شد. همچنین، این چهار سویه باعث افزایش معنی‌دار شاخص‌های رشد گیاه برنج نسبت به گیاهان شاهد شد. نتایج این تحقیق نشان داد که قارچ‌های اندوفیت برنج می‌توانند به‌عنوان عوامل کنترل زیستی بیماری‌ پوسیدگی طوقه و افزاینده رشد برنج با هدف کاربردی نمودن آن‌ها در تحقیقات آتی مورد توجه قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

Biological control of rice Bakanae disease caused by using some endophytic fungi

نویسندگان [English]

  • Narjes Khatoon Ramesh 1
  • Shahram Naeimi 2
  • Saeed Rezaee 3
  • Khalil-Berdi Fotouhifar 4

1 Department of Plant Protection, College of Agriculture Sciences and Food Industries , Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extention Organization (AREEO), Tehran, Iran

3 Department of Plant Protection, College of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

چکیده [English]

The objective of the current study was to isolate and identify the fungal endophytes of rice as the effective and safe alternatives to use indtead of  chemical fungicides for controlling rice Bakanae disease caused by Fusarium fujikuroi. Fungal endophytes obtained from leaf, stem, sheath and root samples of rice plants in paddy fields. The fungal isolates screened against aggressive strain of F. fujikuroi F257in dual culture tests and the most effective endophytes were selected for greenhouse experiments. Rice seedlings were treated with the fungal endophytes and F. fujikuroi F257 isolate. Disease incidence and growth parameters were measured. The lowest Bakanae incidences were recorded using Chaetomium globosum NR-R688 and C. globosum NR-SH321 strains as 2% (with 97.4% disease control) and by NR-L243 Penicillium sp. and Fusarium sp. NR-L645 as 6% (with 92.3% disease control). In addition, these four fungal endophytes significantly promoted the growth parameters of rice seedlings compared to the control. According to the results of this study, fungal endophytes of rice could be applied as the potential biocontrol agents of Bakanae disease and plant growth promoter, but more research is needed for developing them in the future.

کلیدواژه‌ها [English]

  • Biocontrol
  • Fusarium
  • growth parameters
  • nursery
  • seedling blight
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