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القأ مقاومت به بیماری پژمردگی فوزاریومی گوجه‌فرنگی از طریق افزایش در تولید آنزیم‌های آنتی‌اکسیدانی و ژن‌های مرتبط با بیماری‌زایی با استفاده از متیل جاسمونات

نوع مقاله : بیماری‌شناسی گیاهی

نویسندگان

1 Department of Biology, Faculty of استاد دانشگاه یزد Science, Yazd University, Safaieh, 89195-742 Yazd

2 گروه زراعت دانشگاه پیام نور یزد

3 گروه زیست شناسی دانشکده علوم دانشگاه یزد

4 دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشکده منابع طبیعی و کویر شناسی دانشگاه یزد

5 کارشناس آزمایشگاه گروه زیست شناسی دانشکده علوم دانشگاه یزد

چکیده

د
در این تحقیق امکان القای مقاومت سیستمیک به بیماری پژمردگی فوزاریومی ناشی از Fusarium oxysporum fsp. lycopersisi با استفاده از متیل جاسمونات در دو رقم گوجه‌فرنگی ترمه و کاپیتان بررسی شد. نهال‌های گوجه فرنگی با سه غلظت 2/0، 1/0 و صفر در مرحله 2-4 برگی با روش اسپری دستی تیمار و در مرحله 6 برگی با استفاده از عامل بیمارگر، بیماری‌زایی صورت گرفت. سطح بیان سه ژن npr1، pds و eds1، فعالیت آنزیم‌های کاتالاز، پراکسیداز و پلی فنل اکسیداز و میزان فنل کل به‌ترتیب با استفاده از روش بررسی بیان ژن در زمان واقعی و طیف سنجی انجام گردید. نتایج این تحقیق نشان داد که فعالیت آنزیم ها در ارقام و بازه‌های زمانی متغییر می‌باشد. بیشترین میزان فعالیت آنزیم ها در رقم ترمه تحت تنش بیماری مشاهده شد. بیشترین و کمترین سطح بیان به‌ترتیب برای ژن‌های npr1 و pds اتفاق افتاد. نتایج حاصل از این تحقیق حاکی از آن است که متیل جاسمونات قادر به‌ القا مقاومت در گوجه فرنگی بر علیه بیماری پژمردگی فوزاریومی از طریق تغییر در فعالیت‌های بیوشیمیایی و مولکولی می‌باشد.
 

کلیدواژه‌ها

عنوان مقاله [English]

Induced resistance to fusarium tomato wilting disease throught increase of antioxidant enzymes and pathogenesis –related genes using MethylJasmonat

نویسندگان [English]

  • masoud Golesrtani 2
  • Mohammad Rezaa Sarafraz ardakani 3
  • benyamin Kazempour 4
  • marziyeh Tahei 5

2 Peyamnoor University of Yazd

3 Department of Biology, Faculty of Science, Yazd University

4 3- MSc student of Biotechnology, Department of Biotechnology, Faculty of Natural Resources of Desert , Yazd niversity

5 1- Department of Biology, Faculty of Science, Yazd University

چکیده [English]

In this research, possibility of induced systemic resistance in tow tomato cultivars Termeh and Capitan was assayed by exogenous application of methyl jasmonat (MeJA). Tomato plants were treated with methyl jasmonat at concentration of 0, 0.1 and 0.2 mM by spraying at 2-4 leaf stage and then were inoculated withpathogen agent at 6- leaf stages. Gene expression level of npr1, pds and eds1 genes and catalase, peroxidase, polyphenol oxidase activity and total phenol continent was investigated using qRT-PCR and spectrophotometery methods, repectively. The results of this research showed that enzyme activity was variable in tested cultivars and interval times after inoculation. The highest enzyme activity was observed in Termeh cultivar under disease stress. The highest and lowest expression level was occured in npr1 and pds genes, respectively. These results indicated that methyl jasmonat is able to iduces resistance in tomato against wilting disease throught change on bio-chemical and molecular activities.
 

کلیدواژه‌ها [English]

  • Acquired resistance
  • gene expression
  • methyl jasmonat
  • transcription factors
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آفات و بیماری‌های گیاهی
دوره 88، شماره 2 - شماره پیاپی 111
اسفند 1399
صفحه 275-284
فایل ها
هم رسانی
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